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Journal of Bacteriology, February 1999, p. 1319-1323, Vol. 181, No. 4
Institute for Veterinary Bacteriology,
University of Berne, CH-3012 Berne, Switzerland
Received 6 August 1998/Accepted 30 November 1998
A new insertion sequence, IS1634, has been identified
in Mycoplasma mycoides subsp. mycoides
small-colony type (SC). IS1634 shows structural and
functional similarities to IS1549 of Mycobacterium smegmatis and with it seems to form a new class or family of
insertion sequences. IS1634 has a size of 1,872 bp,
including two 13-bp terminal inverted repeats. It contains an open
reading frame (ORF) encoding a product of 533 amino acids which shows
similarity to the transposase of IS1549 and to a lesser
extent to the transposases of IS elements of the IS4
family. IS1634 is present at about 30 copies in the genome
of all 22 different field strains of M. mycoides subsp. mycoides SC tested.
Characteristic of IS1634 are the long and variable-length
direct repeats at the sites of insertion which were found to reach up
to about 500 bp. IS1634 is specific to M. mycoides subsp. mycoides SC and is not present
in any of the other members of the M. mycoides
cluster. Neither was it found in other closely related
Mycoplasma species of ruminants.
0021-9193/99/$04.00+0
Copyright © 1999, American Society for Microbiology. All rights reserved.
IS1634, a Novel Insertion Element Creating Long,
Variable-Length Direct Repeats Which Is Specific for Mycoplasma
mycoides subsp. mycoides Small-Colony
Type
*
Corresponding author. Mailing address: Institute for
Veterinary Bacteriology, University of Berne, Länggass-Strasse
122, CH-3012 Berne, Switzerland. Phone: 41 31 631 2484. Fax: 41 31 631 2634. E-mail: jfrey{at}vbi.unibe.ch.
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