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Journal of Bacteriology, February 1999, p. 1334-1337, Vol. 181, No. 4
Ru
Received 28 April 1998/Accepted 5 December 1998
The recB268::Tn10 mutation was
introduced into the HfrH strain of Escherichia coli.
Compared with recB F
0021-9193/99/$04.00+0
Copyright © 1999, American Society for Microbiology. All rights reserved.
Specific Effects of a recB Mutation on
the HfrH Strain of Escherichia coli
and
eljko
Trgov
evi
*
er
Bo
kovi
Institute, 10001 Zagreb, Croatia
and recB
F+ cells, the viability of this mutant strain was much
lower. Compared with wild-type HfrH, the recB derivative
donated much shorter fragments of its chromosome to the recipient. It
is suggested that the recB gene product (i.e., RecBCD
enzyme) participates in Hfr transfer.
*
Corresponding author. Mailing address: Department of
Molecular Genetics, Ru
er Bo
kovi
Institute,
Bijeni
ka 54, P.O. Box 1016, 10001 Zagreb, Croatia. Phone:
385-1-4561099. Fax: 385-1-4561177. E-mail:
dermic{at}rudjer.irb.hr.
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