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Journal of Bacteriology, February 1999, p. 1334-1337, Vol. 181, No. 4
0021-9193/99/$04.00+0
Copyright © 1999, American Society for Microbiology. All rights reserved.

Specific Effects of a recB Mutation on the HfrH Strain of Escherichia coli

Damir Dermic' and Zeljko Trgovcevic'*

Ruder Boskovic' Institute, 10001 Zagreb, Croatia

Received 28 April 1998/Accepted 5 December 1998

The recB268::Tn10 mutation was introduced into the HfrH strain of Escherichia coli. Compared with recB F- and recB F+ cells, the viability of this mutant strain was much lower. Compared with wild-type HfrH, the recB derivative donated much shorter fragments of its chromosome to the recipient. It is suggested that the recB gene product (i.e., RecBCD enzyme) participates in Hfr transfer.

* Corresponding author. Mailing address: Department of Molecular Genetics, Ruder Boskovic' Institute, Bijenicka 54, P.O. Box 1016, 10001 Zagreb, Croatia. Phone: 385-1-4561099. Fax: 385-1-4561177. E-mail: dermic{at}rudjer.irb.hr.

Journal of Bacteriology, February 1999, p. 1334-1337, Vol. 181, No. 4
0021-9193/99/$04.00+0
Copyright © 1999, American Society for Microbiology. All rights reserved.


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