This Article
Right arrow Full Text
Right arrow Full Text (PDF)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrowReprints and Permissions
Right arrow Copyright Information
Right arrow Books from ASM Press
Right arrow MicrobeWorld
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Kertesz, M. A.
Right arrow Articles by Wüest, T.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Kertesz, M. A.
Right arrow Articles by Wüest, T.

 Previous Article  |  Next Article 

Journal of Bacteriology, March 1999, p. 1464-1473, Vol. 181, No. 5
0021-9193/99/$04.00+0
Copyright © 1999, American Society for Microbiology. All rights reserved.

A Novel Reduced Flavin Mononucleotide-Dependent Methanesulfonate Sulfonatase Encoded by the Sulfur-Regulated msu Operon of Pseudomonas aeruginosa

Michael A. Kertesz,1,* Karen Schmidt-Larbig,2 and Thomas Wüest1,dagger

Institute of Microbiology, Swiss Federal Institute of Technology, ETH-Zentrum, CH-8092 Zurich, Switzerland,1 and Klinische Forschergruppe, Zentrum Biochemie, Medizinische Hochschule Hannover, D-30623 Hannover, Germany2

Received 1 October 1998/Accepted 16 December 1998

When Pseudomonas aeruginosa is grown with organosulfur compounds as sulfur sources, it synthesizes a set of proteins whose synthesis is repressed in the presence of sulfate, cysteine, or thiocyanate (so-called sulfate starvation-induced proteins). The gene encoding one of these proteins, PA13, was isolated from a cosmid library of P. aeruginosa PAO1 and sequenced. It encoded a 381-amino-acid protein that was related to several reduced flavin mononucleotide (FMNH2)-dependent monooxygenases, and it was the second in an operon of three genes, which we have named msuEDC. The MsuD protein catalyzed the desulfonation of alkanesulfonates, requiring oxygen and FMNH2 for the reaction, and showed highest activity with methanesulfonate. MsuE was an NADH-dependent flavin mononucleotide (FMN) reductase, which provided reduced FMN for the MsuD enzyme. Expression of the msu operon was analyzed with a transcriptional msuD::xylE fusion and was found to be repressed in the presence of sulfate, sulfite, sulfide, or cysteine and derepressed during growth with methionine or alkanesulfonates. Growth with methanesulfonate required an intact cysB gene, and the msu operon is therefore part of the cys regulon, since sulfite utilization was found to be CysB independent in this species. Measurements of msuD::xylE expression in cysN and cysI genetic backgrounds showed that sulfate, sulfite, and sulfide or cysteine play independent roles in negatively regulating msu expression, and sulfonate utilization therefore appears to be tightly regulated.

* Corresponding author. Mailing address: Institute of Microbiology, Swiss Federal Institute of Technology, ETH-Zentrum, CH-8092 Zürich, Switzerland. Phone: 41-1-632 33 57. Fax: 41-1-632 11 48. E-mail: kertesz{at}micro.biol.ethz.ch.

dagger Present address: Institut für Zellbiologie und Immunologie, Universität Stuttgart, 70569 Stuttgart, Germany.

Journal of Bacteriology, March 1999, p. 1464-1473, Vol. 181, No. 5
0021-9193/99/$04.00+0
Copyright © 1999, American Society for Microbiology. All rights reserved.


This article has been cited by other articles:

  • Tralau, T., Vuilleumier, S., Thibault, C., Campbell, B. J., Hart, C. A., Kertesz, M. A. (2007). Transcriptomic Analysis of the Sulfate Starvation Response of Pseudomonas aeruginosa. J. Bacteriol. 189: 6743-6750 [Abstract] [Full Text]  
  • Endoh, T., Habe, H., Yoshida, T., Nojiri, H., Omori, T. (2003). A CysB-regulated and {sigma}54-dependent regulator, SfnR, is essential for dimethyl sulfone metabolism of Pseudomonas putida strain DS1. Microbiology 149: 991-1000 [Abstract] [Full Text]  
  • Baxter, N. J., Scanlan, J., De Marco, P., Wood, A. P., Murrell, J. C. (2002). Duplicate Copies of Genes Encoding Methanesulfonate Monooxygenase in Marinosulfonomonas methylotropha Strain TR3 and Detection of Methanesulfonate Utilizers in the Environment. Appl. Environ. Microbiol. 68: 289-296 [Abstract] [Full Text]  
  • Coppee, J.-Y., Auger, S., Turlin, E., Sekowska, A., Le Caer, J.-P., Labas, V., Vagner, V., Danchin, A., Martin-Verstraete, I. (2001). Sulfur-limitation-regulated proteins in Bacillus subtilis: a two-dimensional gel electrophoresis study. Microbiology 147: 1631-1640 [Abstract] [Full Text]  
  • Kahnert, A., Vermeij, P., Wietek, C., James, P., Leisinger, T., Kertesz, M. A. (2000). The ssu Locus Plays a Key Role in Organosulfur Metabolism in Pseudomonas putida S-313. J. Bacteriol. 182: 2869-2878 [Abstract] [Full Text]  
  • Hummerjohann, J., Laudenbach, S., Rétey, J., Leisinger, T., Kertesz, M. A. (2000). The Sulfur-Regulated Arylsulfatase Gene Cluster of Pseudomonas aeruginosa, a New Member of the cys Regulon. J. Bacteriol. 182: 2055-2058 [Abstract] [Full Text]  
  • Galán, B., Díaz, E., Prieto, M. A., García, J. L. (2000). Functional Analysis of the Small Component of the 4-Hydroxyphenylacetate 3-Monooxygenase of Escherichia coli W: a Prototype of a New Flavin:NAD(P)H Reductase Subfamily. J. Bacteriol. 182: 627-636 [Abstract] [Full Text]  
  • van der Ploeg, J. R., Iwanicka-Nowicka, R., Bykowski, T., Hryniewicz, M. M., Leisinger, T. (1999). The Escherichia coli ssuEADCB Gene Cluster Is Required for the Utilization of Sulfur from Aliphatic Sulfonates and Is Regulated by the Transcriptional Activator Cbl. J. Biol. Chem. 274: 29358-29365 [Abstract] [Full Text]  
  • Eichhorn, E., van der Ploeg, J. R., Leisinger, T. (1999). Characterization of a Two-component Alkanesulfonate Monooxygenase from Escherichia coli. J. Biol. Chem. 274: 26639-26646 [Abstract] [Full Text]  
  • Vermeij, P., Kertesz, M. A. (1999). Pathways of Assimilative Sulfur Metabolism in Pseudomonas putida. J. Bacteriol. 181: 5833-5837 [Abstract] [Full Text]  
  • Hogan, D. A., Auchtung, T. A., Hausinger, R. P. (1999). Cloning and Characterization of a Sulfonate/alpha -Ketoglutarate Dioxygenase from Saccharomyces cerevisiae. J. Bacteriol. 181: 5876-5879 [Abstract] [Full Text]  


Copyright © 2009 by the American Society for Microbiology.   For an alternate route to Journals.ASM.org, visit: http://intl-journals.asm.org | More Info»