This Article
Right arrow Full Text
Right arrow Full Text (PDF)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrowReprints and Permissions
Right arrow Copyright Information
Right arrow Books from ASM Press
Right arrow MicrobeWorld
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Napoli, A.
Right arrow Articles by Ciaramella, M.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Napoli, A.
Right arrow Articles by Ciaramella, M.

 Previous Article  |  Next Article 

Journal of Bacteriology, March 1999, p. 1474-1480, Vol. 181, No. 5
0021-9193/99/$04.00+0
Copyright © 1999, American Society for Microbiology. All rights reserved.

An Lrp-Like Protein of the Hyperthermophilic Archaeon Sulfolobus solfataricus Which Binds to Its Own Promoterdagger

Alessandra Napoli,1 John van der Oost,2 Christoph W. Sensen,3 Robert L. Charlebois,4 Mosé Rossi,1 and Maria Ciaramella1,*

Institute of Protein Biochemistry and Enzymology, Consiglio Nazionale delle Ricerche, 80125 Naples, Italy1; Department of Microbiology, Wageningen Agricultural University, Wageningen 6703 CT, The Netherlands2; and Institute for Marine Biosciences, National Research Council of Canada, Halifax, NS, B3H 3Z1,3 and Department of Biology, University of Ottawa, Ottawa, Ontario K1N 6N54 Canada

Received 20 October 1998/Accepted 21 December 1998

Regulation of gene expression in the domain Archaea, and specifically hyperthermophiles, has been poorly investigated so far. Biochemical experiments and genome sequencing have shown that, despite the prokaryotic cell and genome organization, basal transcriptional elements of members of the domain Archaea (i.e., TATA box-like sequences, RNA polymerase, and transcription factors TBP, TFIIB, and TFIIS) are of the eukaryotic type. However, open reading frames potentially coding for bacterium-type transcription regulation factors have been recognized in different archaeal strains. This finding raises the question of how bacterial and eukaryotic elements interact in regulating gene expression in Archaea. We have identified a gene coding for a bacterium-type transcription factor in the hyperthermophilic archaeon Sulfolobus solfataricus. The protein, named Lrs14, contains a potential helix-turn-helix motif and is related to the Lrp-AsnC family of regulators of gene expression in the class Bacteria. We show that Lrs14, expressed in Escherichia coli, is a highly thermostable DNA-binding protein. Bandshift and DNase I footprint analyses show that Lrs14 specifically binds to multiple sequences in its own promoter and that the region of binding overlaps the TATA box, suggesting that, like the E. coli Lrp, Lrs14 is autoregulated. We also show that the lrs14 transcript is accumulated in the late growth stages of S. solfataricus.


* Corresponding author. Mailing address: Institute of Protein Biochemistry and Enzymology, Consiglio Nazionale delle Ricerche, Via Marconi 10, 80125 Naples, Italy. Phone: 39 081 7257 246. Fax: 39 081 239 6525. E-mail: ciaramel{at}dafne.ibpe.na.cnr.it.

dagger NRCC publication 42281.


Journal of Bacteriology, March 1999, p. 1474-1480, Vol. 181, No. 5
0021-9193/99/$04.00+0
Copyright © 1999, American Society for Microbiology. All rights reserved.



This article has been cited by other articles:

  • Kumarevel, T., Nakano, N., Ponnuraj, K., Gopinath, S. C. B., Sakamoto, K., Shinkai, A., Kumar, P. K. R., Yokoyama, S. (2008). Crystal structure of glutamine receptor protein from Sulfolobus tokodaii strain 7 in complex with its effector L-glutamine: implications of effector binding in molecular association and DNA binding. Nucleic Acids Res 36: 4808-4820 [Abstract] [Full Text]  
  • Abella, M., Rodriguez, S., Paytubi, S., Campoy, S., White, M. F., Barbe, J. (2007). The Sulfolobus solfataricus radA paralogue sso0777 is DNA damage inducible and positively regulated by the Sta1 protein. Nucleic Acids Res 35: 6788-6797 [Abstract] [Full Text]  
  • Kessler, A., Sezonov, G., Guijarro, J. I., Desnoues, N., Rose, T., Delepierre, M., Bell, S. D., Prangishvili, D. (2006). A novel archaeal regulatory protein, Sta1, activates transcription from viral promoters. Nucleic Acids Res 34: 4837-4845 [Abstract] [Full Text]  
  • Peeters, E., Willaert, R., Maes, D., Charlier, D. (2006). Ss-LrpB from Sulfolobus solfataricus Condenses about 100 Base Pairs of Its Own Operator DNA into Globular Nucleoprotein Complexes. J. Biol. Chem. 281: 11721-11728 [Abstract] [Full Text]  
  • Thaw, P., Sedelnikova, S. E., Muranova, T., Wiese, S., Ayora, S., Alonso, J. C., Brinkman, A. B., Akerboom, J., van der Oost, J., Rafferty, J. B. (2006). Structural insight into gene transcriptional regulation and effector binding by the Lrp/AsnC family. Nucleic Acids Res 34: 1439-1449 [Abstract] [Full Text]  
  • Cordone, A., Mauriello, E. M. F., Pickard, D. J., Dougan, G., De Felice, M., Ricca, E. (2005). The lrp Gene and Its Role in Type I Fimbriation in Citrobacter rodentium. J. Bacteriol. 187: 7009-7017 [Abstract] [Full Text]  
  • Xie, Y., Reeve, J. N. (2005). Regulation of Tryptophan Operon Expression in the Archaeon Methanothermobacter thermautotrophicus. J. Bacteriol. 187: 6419-6429 [Abstract] [Full Text]  
  • Salerno, V., Napoli, A., White, M. F., Rossi, M., Ciaramella, M. (2003). Transcriptional response to DNA damage in the archaeon Sulfolobus solfataricus. Nucleic Acids Res 31: 6127-6138 [Abstract] [Full Text]  
  • Fiorentino, G., Cannio, R., Rossi, M., Bartolucci, S. (2003). Transcriptional Regulation of the Gene Encoding an Alcohol Dehydrogenase in the Archaeon Sulfolobus solfataricus Involves Multiple Factors and Control Elements. J. Bacteriol. 185: 3926-3934 [Abstract] [Full Text]  
  • Lee, S.-J., Engelmann, A., Horlacher, R., Qu, Q., Vierke, G., Hebbeln, C., Thomm, M., Boos, W. (2003). TrmB, a Sugar-specific Transcriptional Regulator of the Trehalose/Maltose ABC Transporter from the Hyperthermophilic Archaeon Thermococcus litoralis. J. Biol. Chem. 278: 983-990 [Abstract] [Full Text]  
  • Brinkman, A. B., Bell, S. D., Lebbink, R. J., de Vos, W. M., van der Oost, J. (2002). The Sulfolobus solfataricus Lrp-like Protein LysM Regulates Lysine Biosynthesis in Response to Lysine Availability. J. Biol. Chem. 277: 29537-29549 [Abstract] [Full Text]  
  • Napoli, A., Zivanovic, Y., Bocs, C., Buhler, C., Rossi, M., Forterre, P., Ciaramella, M. (2002). DNA bending, compaction and negative supercoiling by the architectural protein Sso7d of Sulfolobus solfataricus. Nucleic Acids Res 30: 2656-2662 [Abstract] [Full Text]  
  • Dahlke, I., Thomm, M. (2002). A Pyrococcus homolog of the leucine-responsive regulatory protein, LrpA, inhibits transcription by abrogating RNA polymerase recruitment. Nucleic Acids Res 30: 701-710 [Abstract] [Full Text]  
  • She, Q., Singh, R. K., Confalonieri, F., Zivanovic, Y., Allard, G., Awayez, M. J., Chan-Weiher, C. C.-Y., Clausen, I. G., Curtis, B. A., De Moors, A., Erauso, G., Fletcher, C., Gordon, P. M. K., Heikamp-de Jong, I., Jeffries, A. C., Kozera, C. J., Medina, N., Peng, X., Thi-Ngoc, H. P., Redder, P., Schenk, M. E., Theriault, C., Tolstrup, N., Charlebois, R. L., Doolittle, W. F., Duguet, M., Gaasterland, T., Garrett, R. A., Ragan, M. A., Sensen, C. W., Van der Oost, J. (2001). The complete genome of the crenarchaeon Sulfolobus solfataricus P2. Proc. Natl. Acad. Sci. USA 10.1073/pnas.141222098v1 [Abstract] [Full Text]  
  • Hanzelka, B. L., Darcy, T. J., Reeve, J. N. (2001). TFE, an Archaeal Transcription Factor in Methanobacterium thermoautotrophicum Related to Eucaryal Transcription Factor TFIIE{alpha}. J. Bacteriol. 183: 1813-1818 [Abstract] [Full Text]  
  • Enoru-Eta, J., Gigot, D., Thia-Toong, T.-L., Glansdorff, N., Charlier, D. (2000). Purification and Characterization of Sa-Lrp, a DNA-Binding Protein from the Extreme Thermoacidophilic Archaeon Sulfolobus acidocaldarius Homologous to the Bacterial Global Transcriptional Regulator Lrp. J. Bacteriol. 182: 3661-3672 [Abstract] [Full Text]  
  • Bell, S. D., Jackson, S. P. (2000). Mechanism of Autoregulation by an Archaeal Transcriptional Repressor. J. Biol. Chem. 275: 31624-31629 [Abstract] [Full Text]  
  • Brinkman, A. B., Dahlke, I., Tuininga, J. E., Lammers, T., Dumay, V., de Heus, E., Lebbink, J. H. G., Thomm, M., de Vos, W. M., van der Oost, J. (2000). An Lrp-like Transcriptional Regulator from the Archaeon Pyrococcus furiosus Is Negatively Autoregulated. J. Biol. Chem. 275: 38160-38169 [Abstract] [Full Text]  
  • Napoli, A., Kvaratskelia, M., White, M. F., Rossi, M., Ciaramella, M. (2001). A Novel Member of the Bacterial-Archaeal Regulator Family Is a Nonspecific DNA-binding Protein and Induces Positive Supercoiling. J. Biol. Chem. 276: 10745-10752 [Abstract] [Full Text]  
  • She, Q., Singh, R. K., Confalonieri, F., Zivanovic, Y., Allard, G., Awayez, M. J., Chan-Weiher, C. C.-Y., Clausen, I. G., Curtis, B. A., De Moors, A., Erauso, G., Fletcher, C., Gordon, P. M. K., Heikamp-de Jong, I., Jeffries, A. C., Kozera, C. J., Medina, N., Peng, X., Thi-Ngoc, H. P., Redder, P., Schenk, M. E., Theriault, C., Tolstrup, N., Charlebois, R. L., Doolittle, W. F., Duguet, M., Gaasterland, T., Garrett, R. A., Ragan, M. A., Sensen, C. W., Van der Oost, J. (2001). The complete genome of the crenarchaeon Sulfolobus solfataricus P2. Proc. Natl. Acad. Sci. USA 98: 7835-7840 [Abstract] [Full Text]