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Journal of Bacteriology, March 1999, p. 1673-1676, Vol. 181, No. 5
0021-9193/99/$04.00+0
Copyright © 1999, American Society for Microbiology. All rights reserved.

Identification of Mechanosensitive Ion Channels in the Cytoplasmic Membrane of Corynebacterium glutamicum

Susanne Ruffert,1 Catherine Berrier,2 Reinhard Krämer,1 and Alexandre Ghazi2,*

Institut für Biochemie, Universität zu Köln, 50674 Köln, Germany,1 and Laboratoire des Biomembranes, ERS 571 du CNRS, Université Paris-Sud, 91405 Orsay, France2

Received 19 October 1998/Accepted 17 December 1998

Patch-clamp experiments performed on membrane fragments of Corynebacterium glutamicum fused into giant liposomes revealed the presence of two different stretch-activated conductances, 600 to 700 pS and 1,200 to 1,400 pS in 0.1 M KCl, that exhibited the same characteristics in terms of kinetics, ion selectivity, and voltage dependence.

* Corresponding author. Mailing address: Laboratoire des Biomembranes, ERS CNRS 571, Bât. 430, Université Paris-Sud, 91405 Orsay, France. Phone: 33 1 69 15 71 94. Fax: 33 1 69 85 37 15. E-mail: alexandre.ghazi{at}biomemb.u-psud.fr.

Journal of Bacteriology, March 1999, p. 1673-1676, Vol. 181, No. 5
0021-9193/99/$04.00+0
Copyright © 1999, American Society for Microbiology. All rights reserved.


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