Journal of Bacteriology, March 1999, p. 1719-1727, Vol. 181, No. 6
0021-9193/99/$04.00+0
Copyright © 1999, American Society for Microbiology. All rights reserved.
Department of Microbiology, Technical University of Denmark, DK-2800 Lyngby, Denmark
Received 8 October 1998/Accepted 9 January 1999
The deoR gene located just upstream the
dra-nupC-pdp operon of Bacillus subtilis
encodes the DeoR repressor protein that negatively regulates the
expression of the operon at the level of transcription. The control
region upstream of the operon was mapped by the use of transcriptional
lacZ fusions. It was shown that all of the cis-acting elements, which were necessary for full DeoR
regulation of the operon, were included in a 141-bp sequence just
upstream of dra. The increased copy number of this control
region resulted in titration of the DeoR molecules of the cell. By
using mutagenic PCR and site-directed mutagenesis techniques, a
palindromic sequence located from position
60 to position
43
relative to the transcription start point was identified as a part of
the operator site for the binding of DeoR. Furthermore, it was shown
that a direct repeat of five nucleotides, which was identical to the 3'
half of the palindrome and was located between the
10 and
35
regions of the dra promoter, might function as a half
binding site involved in cooperative binding of DeoR to the regulatory
region. Binding of DeoR protein to the operator DNA was confirmed by a
gel electrophoresis mobility shift assay. Moreover,
deoxyribose-5-phosphate was shown to be a likely candidate for the true
inducer of the dra-nupC-pdp expression.
This article has been cited by other articles:
| Appl. Environ. Microbiol. | Infect. Immun. | Eukaryot. Cell |
|---|---|---|
| Mol. Cell. Biol. | J. Virol. | Microbiol. Mol. Biol. Rev. |
| ALL ASM JOURNALS |