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Journal of Bacteriology, March 1999, p. 1786-1792, Vol. 181, No. 6
0021-9193/99/$04.00+0
Copyright © 1999, American Society for Microbiology. All rights reserved.

Functional Identification of the Product of the Bacillus subtilis yvaL Gene as a SecG Homologue

Karel H. M. van Wely,1 Jelto Swaving,1 Cees P. Broekhuizen,2 Matthias Rose,3 Wim J. Quax,4 and Arnold J. M. Driessen1,*

Department of Microbiology, Groningen Biomolecular Sciences and Biotechnology Institute, University of Groningen, 9751 NN Haren,1 Genencor International, 2600 AP Delft,2 and Department of Pharmaceutical Biology, University of Groningen, 9713 GZ Groningen,4 The Netherlands, and Institut für Mikrobiologie, Johann-Wolfgang- Goethe-Universität Frankfurt, D 60439 Frankfurt am Main, Germany3

Received 28 September 1998/Accepted 4 January 1999

Protein export in Escherichia coli is mediated by translocase, a multisubunit membrane protein complex with SecA as the peripheral subunit and the SecY, SecE, and SecG proteins as the integral membrane domain. In the gram-positive bacterium Bacillus subtilis, SecA, SecY, and SecE have been identified through genetic analysis. Sequence comparison of the Bacillus chromosome identified a potential homologue of SecG, termed YvaL. A chromosomal disruption of the yvaL gene results in mild cold sensitivity and causes a beta -lactamase secretion defect. The cold sensitivity is exacerbated by overexpression of the secretory protein alpha -amylase, whereas growth and beta -lactamase secretion are restored by coexpression of yvaL or the E. coli secG gene. These results indicate that the yvaL gene codes for a protein that is functionally homologous to SecG.

* Corresponding author. Mailing address: Department of Microbiology, Groningen Biomolecular Sciences and Biotechnology Institute, University of Groningen, Kerklaan 30, 9751 NN Haren, The Netherlands. Phone: 31 50 3632164. Fax: 31 50 3632154. E-mail: a.j.m.driessen{at}biol.rug.nl.

Journal of Bacteriology, March 1999, p. 1786-1792, Vol. 181, No. 6
0021-9193/99/$04.00+0
Copyright © 1999, American Society for Microbiology. All rights reserved.


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