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Journal of Bacteriology, March 1999, p. 1786-1792, Vol. 181, No. 6
0021-9193/99/$04.00+0
Copyright © 1999, American Society for Microbiology. All rights reserved.
Functional Identification of the Product of the Bacillus
subtilis yvaL Gene as a SecG Homologue
Karel H. M.
van
Wely,1
Jelto
Swaving,1
Cees P.
Broekhuizen,2
Matthias
Rose,3
Wim J.
Quax,4 and
Arnold J. M.
Driessen1,*
Department of Microbiology, Groningen
Biomolecular Sciences and Biotechnology Institute, University of
Groningen, 9751 NN Haren,1 Genencor
International, 2600 AP Delft,2 and
Department of Pharmaceutical Biology, University of Groningen,
9713 GZ Groningen,4 The Netherlands, and
Institut für Mikrobiologie,
Johann-Wolfgang- Goethe-Universität Frankfurt, D 60439 Frankfurt am Main, Germany3
Received 28 September 1998/Accepted 4 January 1999
Protein export in Escherichia coli is mediated by
translocase, a multisubunit membrane protein complex with SecA as the
peripheral subunit and the SecY, SecE, and SecG proteins as the
integral membrane domain. In the gram-positive bacterium Bacillus
subtilis, SecA, SecY, and SecE have been identified through
genetic analysis. Sequence comparison of the Bacillus
chromosome identified a potential homologue of SecG, termed YvaL. A
chromosomal disruption of the yvaL gene results in mild
cold sensitivity and causes a
-lactamase secretion defect. The cold
sensitivity is exacerbated by overexpression of the secretory protein
-amylase, whereas growth and
-lactamase secretion are restored by
coexpression of yvaL or the E. coli secG gene.
These results indicate that the yvaL gene codes for a
protein that is functionally homologous to SecG.
*
Corresponding author. Mailing address: Department of
Microbiology, Groningen Biomolecular Sciences and Biotechnology
Institute, University of Groningen, Kerklaan 30, 9751 NN Haren, The
Netherlands. Phone: 31 50 3632164. Fax: 31 50 3632154. E-mail:
a.j.m.driessen{at}biol.rug.nl.
Journal of Bacteriology, March 1999, p. 1786-1792, Vol. 181, No. 6
0021-9193/99/$04.00+0
Copyright © 1999, American Society for Microbiology. All rights reserved.
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