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Journal of Bacteriology, March 1999, p. 1920-1923, Vol. 181, No. 6
0021-9193/99/$04.00+0
Copyright © 1999, American Society for Microbiology. All rights reserved.

Site-Directed Mutagenesis of Loop L3 of Sucrose Porin ScrY Leads to Changes in Substrate Selectivity

Christine Ulmke,1 Jens Kreth,1 Joseph W. Lengeler,1 Wolfram Welte,2 and Kurt Schmid1,*

AG Genetik, FB Biologie/Chemie, Universität Osnabrück, D-49069 Osnabrück,1 and Fakultät für Biologie, Universität Konstanz, D-78457 Konstanz,2 Germany

Received 3 August 1998/Accepted 21 December 1998

The difference in substrate selectivity of the maltodextrin (LamB) and sucrose (ScrY) porins is attributed mainly to differences in loop L3, which is supposed to constrict the lumen of the pores. We show that even a single mutation (D201Y) in loop L3 leads to a narrowing of the substrate range of ScrY to that resembling LamB. In addition, we removed the putative N-terminal coiled-coil structure of ScrY and studied the effect of this deletion on sucrose transport.

* Corresponding author. Mailing address: AG Genetik, FB Biologie/Chemie, Universität Osnabrück, D-49069 Osnabrück, Germany. Phone: 49-541-969-2885. Fax: 49-541-969-2293. E-mail: kschmid{at}gmx.de.

Journal of Bacteriology, March 1999, p. 1920-1923, Vol. 181, No. 6
0021-9193/99/$04.00+0
Copyright © 1999, American Society for Microbiology. All rights reserved.


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