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Journal of Bacteriology, March 1999, p. 1924-1926, Vol. 181, No. 6
0021-9193/99/$04.00+0
Copyright © 1999, American Society for Microbiology. All rights reserved.

Characterization of the Divergent sacBK and sacAR Operons, Involved in Sucrose Utilization by Lactococcus lactis

Evert J. Luesink, Joey D. Marugg, Oscar P. Kuipers,* and Willem M. de Vos

Microbial Ingredients Section, NIZO Food Research, 6710 BA Ede, The Netherlands

Received 17 June 1998/Accepted 13 January 1999

The divergently transcribed sacBK and sacAR operons, which are involved in the utilization of sucrose by Lactococcus lactis NZ9800, were examined by transcriptional and gene inactivation studies. Northern analyses of RNA isolated from cells grown at the expense of different carbon sources revealed three sucrose-inducible transcripts: one of 3.2 kb containing sacB and sacK, a second of 3.4 kb containing sacA and sacR, and a third of 1.8 kb containing only sacR. The inactivation of the sacR gene by replacement recombination resulted in the constitutive transcription of the sacBK and sacAR operons in the presence of different carbon sources, indicating that SacR acts as a repressor of transcription.

* Corresponding author. Mailing address: Microbial Ingredients Section, NIZO Food Research, P.O. Box 20, 6710 BA Ede, The Netherlands. Phone: 31-318-659525. Fax: 31-318-650400. E-mail: kuipers{at}nizo.nl.

Journal of Bacteriology, March 1999, p. 1924-1926, Vol. 181, No. 6
0021-9193/99/$04.00+0
Copyright © 1999, American Society for Microbiology. All rights reserved.


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