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Journal of Bacteriology, March 1999, p. 1944-1946, Vol. 181, No. 6
0021-9193/99/$04.00+0
Copyright © 1999, American Society for Microbiology. All rights reserved.

A Missense Mutation Accounts for the Defect in the Glycerol-3-Phosphate Acyltransferase Expressed in the plsB26 Mutant

Richard J. Heath1 and Charles O. Rock1,2,*

Department of Biochemistry, St Jude Children's Research Hospital, Memphis, Tennessee 38101,1 and Department of Biochemistry, University of Tennessee, Memphis, Tennessee 381632

Received 3 December 1998/Accepted 14 January 1999

The sn-glycerol-3-phosphate acyltransferase (plsB) catalyzes the first step in membrane phospholipid formation. A conditional Escherichia coli mutant (plsB26) has a single missense mutation (G1045A) predicting the expression of an acyltransferase with an Ala349Thr substitution. The PlsB26 protein had a significantly reduced glycerol-3-phosphate acyltransferase specific activity coupled with an elevated Km for glycerol-3-phosphate.

* Corresponding author. Mailing address: Department of Biochemistry, St. Jude Children's Research Hospital, 332 N. Lauderdale, Memphis, TN 38105-2794. Phone: 901-495-3491. Fax: 901-525-8025. E-mail: charles.rock{at}stjude.org.

Journal of Bacteriology, March 1999, p. 1944-1946, Vol. 181, No. 6
0021-9193/99/$04.00+0
Copyright © 1999, American Society for Microbiology. All rights reserved.


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