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Journal of Bacteriology, March 1999, p. 1958-1962, Vol. 181, No. 6
0021-9193/99/$04.00+0
Copyright © 1999, American Society for Microbiology. All rights reserved.

Expression of the Methanobacterium thermoautotrophicum hpt Gene, Encoding Hypoxanthine (Guanine) Phosphoribosyltransferase, in Escherichia coli

Jørgen Sauerdagger and Per Nygaard*

Department of Biological Chemistry, University of Copenhagen, 1307 Copenhagen K, Denmark

Received 27 July 1998/Accepted 23 December 1998

The hpt gene from the archaeon Methanobacterium thermoautotrophicum, encoding hypoxanthine (guanine) phosphoribosyltransferase, was cloned by functional complementation into Escherichia coli. The hpt-encoded amino acid sequence is most similar to adenine phosphoribosyltransferases, but the encoded enzyme has activity only with hypoxanthine and guanine. The synthesis of the recombinant enzyme is apparently limited by the presence of the rare arginine codons AGA and AGG and the rare isoleucine AUA codon on the hpt gene. The recombinant enzyme was purified to apparent homogeneity.

* Corresponding author. Mailing address: Department of Biological Chemistry, Institute of Molecular Biology, University of Copenhagen, Sølvgade 83, 1307 Copenhagen K, Denmark. Phone: 4535322005. Fax: 4535322040. E-mail: Nygaard{at}mermaid.molbio.ku.dk.

dagger Present address: Department of Chemistry, Carlsberg Laboratory, 2500 Copenhagen, Denmark.

Journal of Bacteriology, March 1999, p. 1958-1962, Vol. 181, No. 6
0021-9193/99/$04.00+0
Copyright © 1999, American Society for Microbiology. All rights reserved.


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