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Journal of Bacteriology, April 1999, p. 2118-2123, Vol. 181, No. 7
0021-9193/99/$04.00+0
Copyright © 1999, American Society for Microbiology. All rights reserved.

Isolation of RNase H Genes That Are Essential for Growth of Bacillus subtilis 168

Mitsuhiro Itaya,1,* Akira Omori,1 Shigenori Kanaya,2 Robert J. Crouch,3 Teruo Tanaka,1,dagger and Kanae Kondo1

Mitsubishi-Kasei Institute of Life Sciences, Machida-shi, Tokyo 194-8511,1 and Material and Life Science, Graduate School of Engineering, Osaka University, Osaka 565-0871,2 Japan, and Laboratory of Molecular Genetics, National Institute of Child Health and Human Development, National Institutes of Health, Bethesda, Maryland 20892-27903

Received 29 October 1998/Accepted 28 January 1999

Two genes encoding functional RNase H (EC 3.1.26.4) were isolated from a gram-positive bacterium, Bacillus subtilis 168. Two DNA clones exhibiting RNase H activities both in vivo and in vitro were obtained from a B. subtilis DNA library. One (28.2 kDa) revealed high similarity to Escherichia coli RNase HII, encoded by the rnhB gene. The other (33.9 kDa) was designated rnhC and encodes B. subtilis RNase HIII. The B. subtilis genome has an rnhA homologue, the product of which has not yet shown RNase H activity. Analyses of all three B. subtilis genes revealed that rnhB and rnhC cannot be simultaneously inactivated. This observation indicated that in B. subtilis both the rnhB and rnhC products are involved in certain essential cellular processes that are different from those suggested by E. coli rnh mutation studies. Sequence conservation between the rnhB and rnhC genes implies that both originated from a single ancestral RNase H gene. The roles of bacterial RNase H may be indicated by the single rnhC homologue in the small genome of Mycoplasma species.

* Corresponding author. Mailing address: Mitsubishi-Kasei Institute of Life Sciences, 11 Minamiooya, Machida-shi, Tokyo 194-8511, Japan. Phone: 81-427-24-6254. Fax: 81-427-24-6316. E-mail: ita{at}libra.ls.m-kagaku.co.jp.

dagger Present address: School of Marine Science and Technology, Tokai University, Shimizu, Shizuoka 424-8610, Japan.

Journal of Bacteriology, April 1999, p. 2118-2123, Vol. 181, No. 7
0021-9193/99/$04.00+0
Copyright © 1999, American Society for Microbiology. All rights reserved.


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