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Journal of Bacteriology, April 1999, p. 2279-2285, Vol. 181, No. 7
0021-9193/99/$04.00+0
Copyright © 1999, American Society for Microbiology. All rights reserved.

Genetic Organization of the Escherichia coli K10 Capsule Gene Cluster: Identification and Characterization of Two Conserved Regions in Group III Capsule Gene Clusters Encoding Polysaccharide Transport Functions

Bradley R. Clarke, Rowan Pearce, and Ian S. Roberts*

School of Biological Sciences, University of Manchester, Manchester M13 9PT, United Kingdom

Received 9 November 1998/Accepted 14 January 1999

Analysis of the Escherichia coli K10 capsule gene cluster identified two regions, regions 1 and 3, conserved between different group III capsule gene clusters. Region 1 encodes homologues of KpsD, KpsM, KpsT, and KpsE proteins, and region 3 encodes homologues of the KpsC and KpsS proteins. An rfaH mutation abolished K10 capsule production, suggesting that expression of the K10 capsule was regulated by RfaH in a manner analogous to group II capsule gene clusters. An IS3 element and a phi R73-like prophage, both of which may have played a role in the acquisition of group III capsule gene clusters, were detected flanking the K10 capsule genes.

* Corresponding author. Mailing address: School of Biological Sciences, 1.800 Stopford Building, University of Manchester, Oxford Rd., Manchester M13 9PT, United Kingdom. Phone: 0044-(0)161-275-5601. Fax: 0044-(0)161-275-5656. E-mail: ISROBERT{at}fs1.scg.man.ac.uk.

Journal of Bacteriology, April 1999, p. 2279-2285, Vol. 181, No. 7
0021-9193/99/$04.00+0
Copyright © 1999, American Society for Microbiology. All rights reserved.


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