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Journal of Bacteriology, April 1999, p. 2286-2289, Vol. 181, No. 7
0021-9193/99/$04.00+0
Copyright © 1999, American Society for Microbiology. All rights reserved.

Target Joining of Duplicated Insertion Sequence IS21 Is Assisted by IstB Protein In Vitro

Sergio Schmid,1,2 Bernard Berger,3 and Dieter Haas1,3,*

Mikrobiologisches Institut, Eidgenössische Technische Hochschule, CH-8092 Zürich,1 Ecole d'Ingénieurs du Valais, CH-1950 Sion,2 and Laboratoire de Biologie Microbienne, Université de Lausanne, CH-1015 Lausanne,3 Switzerland

Received 10 September 1998/Accepted 24 January 1999

Tandemly repeated insertion sequence IS21, located on a suicide plasmid, promoted replicon fusion with bacteriophage lambda  in vitro in the presence of ATP. This reaction was catalyzed in a cell extract containing the 45-kDa IstA protein (cointegrase) and the 30-kDa IstB helper protein of IS21 after both proteins had been overproduced in Escherichia coli. Without IstB, replicon fusion was inefficient and did not produce the 4-bp target duplications typical of IS21.

* Corresponding author. Mailing address: Laboratoire de Biologie Microbienne, Université de Lausanne, CH-1015 Lausanne, Switzerland. Phone: 41 21 692 56 31. Fax: 41 21 692 56 35. E-mail: Dieter.Haas{at}lbm.unil.ch.

Journal of Bacteriology, April 1999, p. 2286-2289, Vol. 181, No. 7
0021-9193/99/$04.00+0
Copyright © 1999, American Society for Microbiology. All rights reserved.


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