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Journal of Bacteriology, April 1999, p. 2290-2292, Vol. 181, No. 7
0021-9193/99/$04.00+0
Copyright © 1999, American Society for Microbiology. All rights reserved.

Isolation of an Active Catalytic Core of Streptococcus downei MFe28 GTF-I Glucosyltransferase

Vincent Monchois,1,* Martha Arguello-Morales,2,3 and Roy R. B. Russell1

Department of Oral Biology, The Dental School, University of Newcastle upon Tyne, Newcastle upon Tyne NE2 4BW, United Kingdom,1 and Centre de Bioingénierie Gilbert Durand, UMR CNRS 5504,2 and Laboratoire Associé INRA, DGBA, INSA,3 31077 Toulouse Cedex 4, France

Received 6 October 1998/Accepted 20 January 1999

Truncated variants of GTF-I from Streptococcus downei MFe28 were purified by means of a histidine tag. Sequential deletions showed that the C-terminal domain was not directly involved in the catalytic process but was required for primer activation. A fully active catalytic core of only 100 kDa was isolated.

* Corresponding author. Mailing address: Department of Oral Biology, Dental School, University of Newcastle upon Tyne, Newcastle upon Tyne NE2 4BW, United Kingdom. Phone: (44) 191 222 8391. Fax: (44) 191 222 6137. E-mail: vincent.monchois{at}ncl.ac.uk.

Journal of Bacteriology, April 1999, p. 2290-2292, Vol. 181, No. 7
0021-9193/99/$04.00+0
Copyright © 1999, American Society for Microbiology. All rights reserved.


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