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Journal of Bacteriology, April 1999, p. 2298-2301, Vol. 181, No. 7
0021-9193/99/$04.00+0
Copyright © 1999, American Society for Microbiology. All rights reserved.

Cellular Locations of Pseudomonas syringae pv. syringae HrcC and HrcJ Proteins, Required for Harpin Secretion via the Type III Pathway

Wen-Ling Dengdagger and Hsiou-Chen Huang*

Graduate Institute of Agricultural Biotechnology, National Chung-Hsing University, Taichung 402, Taiwan

Received 6 October 1998/Accepted 22 January 1999

The complete hrp-hrc-hrmA cluster of Pseudomonas syringae pv. syringae 61 encodes 28 polypeptides. A saprophytic bacterium carrying this cluster is capable of secreting HrpZ---a harpin encoded by hrpZ---in an hrp-dependent manner, which suggests that this cluster contains sufficient components to assemble functional type III secretion machinery. Sequence data show that HrcJ and HrcC are putative outer membrane proteins, and nonpolar mutagenesis demonstrates they are all required for HrpZ secretion. In this study, we investigated the cellular localization of the HrcC and HrcJ proteins by Triton solubilization, sucrose-gradient isopycnic centrifugation, and immunogold labeling of the bacterial cell surface. Our results indicate that HrcC is indeed an outer membrane protein and that HrcJ is located between both membranes. Their membrane localization suggests that they might be involved in the formation of a supramolecular structure for protein secretion.

* Corresponding author. Mailing address: Graduate Institute of Agricultural Biotechnology, National Chung-Hsing University, Taichung 402, Taiwan. Phone: 886-4-2852155. Fax: 886-4-2861905. E-mail: hchuang{at}dragon.nchu.edu.tw.

dagger Present address: Department of Plant Pathology, Cornell University, Ithaca, NY 14853-4203.

Journal of Bacteriology, April 1999, p. 2298-2301, Vol. 181, No. 7
0021-9193/99/$04.00+0
Copyright © 1999, American Society for Microbiology. All rights reserved.


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