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Journal of Bacteriology, April 1999, p. 2315-2322, Vol. 181, No. 8
0021-9193/99/$04.00+0
Copyright © 1999, American Society for Microbiology. All rights reserved.

NahW, a Novel, Inducible Salicylate Hydroxylase Involved in Mineralization of Naphthalene by Pseudomonas stutzeri AN10

Rafael Bosch,1,2,* Edward R. B. Moore,2 Elena García-Valdés,1 and Dietmar H. Pieper2

Departament de Biologia, Microbiologia, Universitat de les Illes Balears, and Institut Mediterrani d'Estudis Avançats (CSIC-UIB), 07071, Palma de Mallorca, Spain,1 and Bereich Mikrobiologie, Gesellschaft für Biotechnologische Forschung mbH (GBF), 38124, Braunschweig, Germany2

Received 17 September 1998/Accepted 6 February 1999

Two genes, nahG and nahW, encoding two independent salicylate 1-hydroxylases have been identified in the naphthalene-degrading strain Pseudomonas stutzeri AN10. While nahG resides in the same transcriptional unit as the meta-cleavage pathway genes, forming the naphthalene degradation lower pathway, nahW is situated outside but in close proximity to this transcriptional unit. The nahG and nahW genes of P. stutzeri AN10 are induced and expressed upon incubation with salicylate, and the enzymes that are encoded, NahG and NahW, are involved in naphthalene and salicylate metabolism. Both genes, nahG and nahW, have been cloned in Escherichia coli JM109. The overexpression of these genes yields peptides with apparent molecular masses of 46 kDa (NahG) and 43 kDa (NahW), respectively. Both enzymes exhibit broad substrate specificities and metabolize salicylate, methylsalicylates, and chlorosalicylates. However, the relative rates by which the substituted analogs are transformed differ considerably.

* Corresponding author. Mailing address: Departament de Biologia, Microbiologia, Universitat de les Illes Balears, Carretera Valldemossa, km 7.5, 07071, Palma de Mallorca, Spain. Phone: 34-971-173141. Fax: 34-971-173184. E-mail: dbarbz0{at}clust.uib.es.

Journal of Bacteriology, April 1999, p. 2315-2322, Vol. 181, No. 8
0021-9193/99/$04.00+0
Copyright © 1999, American Society for Microbiology. All rights reserved.


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