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Journal of Bacteriology, April 1999, p. 2459-2464, Vol. 181, No. 8
Department of Biology, Georgia State
University, Atlanta, Georgia 30303,1 and
Laboratoire de Biologie Microbienne, Université de
Lausanne, CH-1015 Lausanne, Switzerland2
Received 2 December 1998/Accepted 10 February 1999
Pseudomonas aeruginosa, when deprived of oxygen,
generates ATP from arginine catabolism by enzymes of the arginine
deiminase pathway, encoded by the arcDABC operon. Under
conditions of low oxygen tension, the transcriptional activator ANR
binds to a site centered 41.5 bp upstream of the arcD
transcriptional start. ANR-mediated anaerobic induction was enhanced
two- to threefold by extracellular arginine. This arginine effect
depended, in trans, on the transcriptional regulator ArgR
and, in cis, on an ArgR binding site centered at
0021-9193/99/$04.00+0
Copyright © 1999, American Society for Microbiology. All rights reserved.
The ArgR Regulatory Protein, a Helper to the
Anaerobic Regulator ANR during Transcriptional Activation of the
arcD Promoter in Pseudomonas aeruginosa
73.5 bp
in the arcD promoter. Binding of purified ArgR protein to
this site was demonstrated by electrophoretic mobility shift assays and
DNase I footprinting. This ArgR recognition site contained a sequence,
5'-TGACGC-3', which deviated in only 1 base from the common
sequence motif 5'-TGTCGC-3' found in other ArgR binding sites of P. aeruginosa. Furthermore, an alignment of all
known ArgR binding sites confirmed that they consist of two directly repeated half-sites. In the absence of ANR, arginine did not induce the
arc operon, suggesting that ArgR alone does not activate
the arcD promoter. According to a model proposed, ArgR
makes physical contact with ANR and thereby facilitates initiation of
arc transcription.
*
Corresponding author. Mailing address: Dean's Office,
Georgia State University, P.O. Box 4038, Atlanta, GA 30302-4038. Phone: (404) 651-1410. Fax: (404) 651-4739. E-mail:
aabdelal{at}gsu.edu.
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