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Journal of Bacteriology, April 1999, p. 2602-2611, Vol. 181, No. 8
0021-9193/99/$04.00+0
Copyright © 1999, American Society for Microbiology. All rights reserved.

cis-Acting Elements Responsible for Low-Temperature-Inducible Expression of the Gene Coding for the Thermolabile Isocitrate Dehydrogenase Isozyme of a Psychrophilic Bacterium, Vibrio sp. Strain ABE-1

Takehiko Sahara, Masahiro Suzuki, Jun-Ichiro Tsuruha, Yasuhiro Takada, and Noriyuki Fukunaga*

Division of Biological Sciences, Graduate School of Science, Hokkaido University, Sapporo 060-0810, Japan

Received 26 May 1998/Accepted 2 February 1999

Transcriptional control of the low-temperature-inducible icdII gene, encoding the thermolabile isocitrate dehydrogenase of a psychrophilic bacterium, Vibrio sp. strain ABE-1, was found to be mediated in part by a transcriptional silencer locating at nucleotide positions -560 to -526 upstream from the transcription start site of icdII. Deletion of the silencer resulted in a 20-fold-increased level of expression of the gene at low temperature (15°C) but not at high temperature (37°C). In addition, a CCAAT sequence located 2 bases upstream of the -35 region was found to be essential for the low-temperature-inducible expression of the gene. By deletion of this sequence, low-temperature-dependent expression of the gene was completely abolished. The ability of the icdII promoter to control the expression of other genes was confirmed by using a fusion gene containing the icdII promoter region and the promoterless icdI open reading frame, which encodes the non-cold-inducible isocitrate dehydrogenase isozyme of Vibrio sp. strain ABE-1. Escherichia coli transformants harboring icdII acquired an ability to grow rapidly at low temperature.

* Corresponding author. Mailing address: Division of Biological Sciences, Graduate School of Science, Hokkaido University, Sapporo 060-0810, Japan. Phone and fax: 81-11-706-2737. E-mail: fukung{at}sci.hokudai.ac.jp.

Journal of Bacteriology, April 1999, p. 2602-2611, Vol. 181, No. 8
0021-9193/99/$04.00+0
Copyright © 1999, American Society for Microbiology. All rights reserved.


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