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Journal of Bacteriology, April 1999, p. 2620-2623, Vol. 181, No. 8
0021-9193/99/$04.00+0
Copyright © 1999, American Society for Microbiology. All rights reserved.

The Lactate-Proton Symport of Saccharomyces cerevisiae Is Encoded by JEN1

Margarida Casal,1 Sandra Paiva,1 Raquel P. Andrade,1 Carlos Gancedo,2 and Cecília Leão1,*

Centro de Ciências do Ambiente, Departamento de Biologia, Universidade do Minho, 4719 Braga Codex, Portugal,1 and Instituto de Investigaciones Biomédicas Alberto Sols, CSIC-UAM, 28029 Madrid, Spain2

Received 23 October 1998/Accepted 22 January 1999

A mutant of Saccharomyces cerevisiae deficient in the lactate-proton symport was isolated. Transformation of the mutant with a yeast genomic library allowed the isolation of the gene JEN1 that restored lactate transport. Disruption of JEN1 abolished uptake of lactate. The results indicate that, under the experimental conditions tested, no other monocarboxylate permease is able to efficiently transport lactate in S. cerevisiae.

* Corresponding author. Mailing address: Centro de Ciências do Ambiente, Departamento de Biologia, Universidade do Minho, Campus de Gualtar, 4719 Braga Codex, Portugal. Phone: 351-53-604310. Fax: 351-53-678980. E-mail: cleao{at}bio.uminho.pt.

Journal of Bacteriology, April 1999, p. 2620-2623, Vol. 181, No. 8
0021-9193/99/$04.00+0
Copyright © 1999, American Society for Microbiology. All rights reserved.


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