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Journal of Bacteriology, April 1999, p. 2643-2647, Vol. 181, No. 8
0021-9193/99/$04.00+0
Copyright © 1999, American Society for Microbiology. All rights reserved.

In Vitro Synthesis of Peptidoglycan Precursors Modified with N-Acetylputrescine by Cyanophora paradoxa Cyanelle Envelope Membranes

Beatrix Pfanzagl and Wolfgang Löffelhardt*

Institut für Biochemie und Molekulare Zellbiologie der Universität Wien und Ludwig-Boltzmann-Forschungsstelle für Biochemie, A-1030 Vienna, Austria

Received 8 December 1998/Accepted 12 February 1999

The photosynthetic organelles (cyanelles) of the protist Cyanophora paradoxa are surrounded by a peptidoglycan wall, modified through amidation with N-acetylputrescine. Cyanelle envelope membrane preparations were shown to catalyze the lipid-linked steps of peptidoglycan biosynthesis as well as the putrescinylation and subsequent acetylation, occurring at the stage of lipid I and/or lipid II.


* Corresponding author. Mailing address: Institut für Biochemie und Molekulare Zellbiologie, Biozentrum der Universität Wien, Dr. Bohrgasse 9, A-1030 Vienna, Austria. Phone: 43-1-4277-52811. Fax: 43-1-4277-9528. E-mail: WL{at}abc.univie.ac.at.


Journal of Bacteriology, April 1999, p. 2643-2647, Vol. 181, No. 8
0021-9193/99/$04.00+0
Copyright © 1999, American Society for Microbiology. All rights reserved.



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