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Journal of Bacteriology, April 1999, p. 2648-2651, Vol. 181, No. 8
0021-9193/99/$04.00+0
Copyright © 1999, American Society for Microbiology. All rights reserved.

Bordetella pertussis waaA Encodes a Monofunctional 2-Keto-3-Deoxy-D-manno-Octulosonic Acid Transferase That Can Complement an Escherichia coli waaA Mutation

Tomoko Isobe,1 Kimberley A. White,2 Andrew G. Allen,1 Michael Peacock,1 Christian R. H. Raetz,2 and Duncan J. Maskell1,*

The Centre for Veterinary Science, Department of Clinical Veterinary Medicine, University of Cambridge, Cambridge CB3 OES, United Kingdom,1 and Department of Biochemistry, Duke University Medical Center, Durham, North Carolina 277102

Received 18 November 1998/Accepted 4 February 1999

Bordetella pertussis lipopolysaccharide (LPS) contains a single 2-keto-3-deoxy-D-manno-octulosonic acid (Kdo) residue, whereas LPS from Escherichia coli contains at least two. Here we report that B. pertussis waaA encodes an enzyme capable of transferring only a single Kdo during the biosynthesis of LPS and that this activity is sufficient to complement an E. coli waaA mutation.

* Corresponding author. Mailing address: The Centre for Veterinary Science, Department of Clinical Veterinary Medicine, University of Cambridge, Madingley Rd., Cambridge CB3 0ES, United Kingdom. Phone: 44 1223 339868. Fax: 44 1223 337610. E-mail: djm47{at}cam.ac.uk.

Journal of Bacteriology, April 1999, p. 2648-2651, Vol. 181, No. 8
0021-9193/99/$04.00+0
Copyright © 1999, American Society for Microbiology. All rights reserved.


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