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Journal of Bacteriology, April 1999, p. 2659-2662, Vol. 181, No. 8
0021-9193/99/$04.00+0
Copyright © 1999, American Society for Microbiology. All rights reserved.

Sequence Analysis of the Four Plasmid Genes Required To Produce the Circular Peptide Antibiotic Microcin J25

José O. Solbiati,1 Mirella Ciaccio,1 Ricardo N. Farías,1 José E. González-Pastor,2 Felipe Moreno,2 and Raúl A. Salomón1,*

Departamento de Bioquímica de la Nutrición, Instituto Superior de Investigaciones Biológicas (Consejo Nacional de Investigaciones Científicas y Técnicas---Universidad Nacional de Tucumán) and Instituto de Química Biológica "Dr. Bernabé Bloj," 4000 San Miguel de Tucumán, Argentina,1 and Unidad de Genética Molecular, Hospital Ramón y Cajal, 28034 Madrid, Spain2

Received 25 January 1999/Accepted 10 February 1999

A 4.8-kb plasmid region carrying the four genes mcjABCD necessary for production of and immunity to the cyclic peptide antibiotic microcin J25 (MccJ25) has been sequenced. mcjA encodes the primary structure of MccJ25 as a precursor endowed with an N-terminal extension of 37 amino acids. The products of mcjB and mcjC are thought to be involved in microcin maturation, which implies cleavage of McjA and head-tail linkage of the 21-residue pro-MccJ25. The predicted McjD polypeptide, which is highly similar to several ABC exporters, was found to be required for MccJ25 secretion, thus explaining its ability to confer immunity to MccJ25.

* Corresponding author. Mailing address: Departamento de Bioquímica de la Nutrición, INSIBIO, Chacabuco 461, 4000 San Miguel de Tucumán, Argentina. Phone: (54) (381) 4248921. Fax: (54) (381) 4248025. E-mail: salomon{at}unt.edu.ar.

Journal of Bacteriology, April 1999, p. 2659-2662, Vol. 181, No. 8
0021-9193/99/$04.00+0
Copyright © 1999, American Society for Microbiology. All rights reserved.


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