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Journal of Bacteriology, April 1999, p. 2663-2665, Vol. 181, No. 8
0021-9193/99/$04.00+0
Copyright © 1999, American Society for Microbiology. All rights reserved.

Localization of Escherichia coli rpoC Mutations That Affect RNA Polymerase Assembly and Activity at High Temperature

Eduard C. Nedea,1 Dmitriy Markov,1,2 Tatyana Naryshkina,1 and Konstantin Severinov1,*

Waksman Institute and Department of Genetics, Rutgers, The State University, Piscataway, New Jersey 08854,1 and Institute of Molecular Genetics, Russian Academy of Sciences, Moscow, Russia2

Received 27 January 1999/Accepted 12 February 1999

We localized five rpoC (beta ') mutations affecting Escherichia coli RNA polymerase assembly. The Ts4, XH56, and R120 mutations changed beta ' residues conserved throughout eubacteria; the JE10092 mutation occurred in the hypervariable region; rpoC1 (TsX) changed a universally conserved residue and corresponds to yeast rpb1-1. Thus, distinct, predominantly conserved beta ' residues participate in interactions holding RNA polymerase together.


* Corresponding author. Mailing address: Waksman Institute, 190 Frelinghuysen Rd., Piscataway, NJ 08854. Phone: (732) 445-6095. Fax: (732) 445-5735. E-mail: severik{at}waksman.rutgers.edu.


Journal of Bacteriology, April 1999, p. 2663-2665, Vol. 181, No. 8
0021-9193/99/$04.00+0
Copyright © 1999, American Society for Microbiology. All rights reserved.



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