Journal of Bacteriology, April 1999, p. 2663-2665, Vol. 181, No. 8
Waksman Institute and Department of Genetics,
Rutgers, The State University, Piscataway, New Jersey
08854,1 and Institute of Molecular Genetics,
Russian Academy of Sciences, Moscow, Russia2
Received 27 January 1999/Accepted 12 February 1999
We localized five rpoC (
0021-9193/99/$04.00+0
Copyright © 1999, American Society for Microbiology. All rights reserved.
Localization of Escherichia coli rpoC Mutations That
Affect RNA Polymerase Assembly and Activity at High
Temperature
') mutations affecting
Escherichia coli RNA polymerase assembly. The Ts4, XH56,
and R120 mutations changed
' residues conserved throughout
eubacteria; the JE10092 mutation occurred in the hypervariable
region; rpoC1 (TsX) changed a universally conserved residue
and corresponds to yeast rpb1-1. Thus, distinct,
predominantly conserved
' residues participate in interactions
holding RNA polymerase together.
*
Corresponding author. Mailing address: Waksman
Institute, 190 Frelinghuysen Rd., Piscataway, NJ 08854. Phone: (732)
445-6095. Fax: (732) 445-5735. E-mail:
severik{at}waksman.rutgers.edu.
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