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Journal of Bacteriology, May 1999, p. 2703-2709, Vol. 181, No. 9
Unidad de Microbiología, Centro de
Biología Molecular y Celular, Universidad Miguel
Hernández, Campus de San Juan, 03550 San Juan, Alicante,
Spain
Received 28 December 1998/Accepted 3 March 1999
The molecular microevolution of the 23S rRNA gene (rrl)
plus the spacer downstream has been studied by sequencing of different operons from some representative strains of the Escherichia
coli ECOR collection. The rrl gene was fully
sequenced in six strains showing a total of 67 polymorphic sites, a
level of variation per nucleotide similar to that found for the 16S
rRNA gene (rrs) in a previous study. The size of the gene
was highly conserved (2902 to 2905 nucleotides). Most polymorphic sites
were clustered in five secondary-structure helices. Those regions in a
large number of operons were sequenced, and several variations were found. Sequences of the same helix from two different strains were
often widely divergent, and no intermediate forms existed. Intercistronic variability was detected, although it seemed to be lower
than for the rrs gene. The presence of two characteristic sequences was determined by PCR analysis throughout all of the strains
of the ECOR collection, and some correlations with the multilocus
enzyme electrophoresis clusters were detected. The mode of variation of
the rrl gene seems to be quite similar to that of the
rrs gene. Homogenization of the gene families and transfer
of sequences from different clonal lines could explain this pattern of
variation detected; perhaps these factors are more relevant to
evolution than single mutation. The spacer region between the 23S and
5S rRNA genes exhibited a highly polymorphic region, particularly at
the 3' end.
0021-9193/99/$04.00+0
Copyright © 1999, American Society for Microbiology. All rights reserved.
Intraspecific Diversity of the 23S rRNA Gene and
the Spacer Region Downstream in Escherichia coli
and
*
Corresponding author. Mailing address: Unidad de
Microbiología, Centro de Biología Molecular y Celular,
Universidad Miguel Hernández, Campus de San Juan, Apartado 18, 03550 San Juan, Alicante, Spain. Phone: 965919451. Fax: 965919457. E-mail: FRVALERA{at}UMH.ES.
Present address: Departamento de Biotecnología, Universidad
de Alicante, E-03080 Alicante, Spain.
Journal of Bacteriology, May 1999, p. 2703-2709, Vol. 181, No. 9
0021-9193/99/$04.00+0
Copyright © 1999, American Society for Microbiology. All rights reserved.
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