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Journal of Bacteriology, May 1999, p. 2947-2952, Vol. 181, No. 9
0021-9193/99/$04.00+0
Copyright © 1999, American Society for Microbiology. All rights reserved.

Carboxy-Terminal Processing of the Large Subunit of [Fe] Hydrogenase from Desulfovibrio desulfuricans ATCC 7757

E. Claude Hatchikian,1,* Valérie Magro,1 Nicole Forget,1 Yvain Nicolet,2 and Juan C. Fontecilla-Camps2

Unité de Bioénergétique et Ingéniérie des Protéines, IBSM, CNRS, 13402 Marseilles Cedex 20,1 and Laboratoire de Cristallographie et de Cristallogénèse des Protéines, Institut de Biologie Structurale Jean-Pierre Ebel, CEA-CNRS, 38027 Grenoble Cedex 1,2 France

Received 9 December 1998/Accepted 12 February 1999

hydA and hydB, the genes encoding the large (46-kDa) and small (13.5-kDa) subunits of the periplasmic [Fe] hydrogenase from Desulfovibrio desulfuricans ATCC 7757, have been cloned and sequenced. The deduced amino acid sequence of the genes product showed complete identity to the sequence of the well-characterized [Fe] hydrogenase from the closely related species Desulfovibrio vulgaris Hildenborough (G. Voordouw and S. Brenner, Eur. J. Biochem. 148:515-520, 1985). The data show that in addition to the well-known signal peptide preceding the NH2 terminus of the mature small subunit, the large subunit undergoes a carboxy-terminal processing involving the cleavage of a peptide of 24 residues, in agreement with the recently reported data on the three-dimensional structure of the enzyme (Y. Nicolet, C. Piras, P. Legrand, E. C. Hatchikian, and J. C. Fontecilla-Camps, Structure 7:13-23, 1999). We suggest that this C-terminal processing is involved in the export of the protein to the periplasm.

* Corresponding author. Mailing address: Unité de Bioénergétique et Ingéniérie des Protéines, IBSM, CNRS, 31, Chemin Joseph Aiguier, 13402 Marseilles Cedex 20, France. Phone: 04 91 16 41 45. Fax: 04 91 16 45 78. E-mail: hatch{at}ibsm.cnrs-mrs.fr.

Journal of Bacteriology, May 1999, p. 2947-2952, Vol. 181, No. 9
0021-9193/99/$04.00+0
Copyright © 1999, American Society for Microbiology. All rights reserved.


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