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Journal of Bacteriology, May 1999, p. 2973-2978, Vol. 181, No. 9
0021-9193/99/$04.00+0
Copyright © 1999, American Society for Microbiology. All rights reserved.

The MobA-Linked Primase Is the Only Replication Protein of R1162 Required for Conjugal Mobilization

Dorian Henderson and Richard Meyer*

Department of Microbiology and Institute for Cellular and Molecular Biology, University of Texas, Austin, Texas 78712.

Received 21 December 1998/Accepted 1 March 1999

Cells newly transformed with plasmid R1162 DNA were used as donors in conjugal matings to determine if the plasmid replication genes are necessary for transfer. An intact system for vegetative replication is not required for transfer at normal frequency, but the plasmid primase, in the form linked to the nickase, must be present in donor cells.

* Corresponding author. Mailing address: Department of Microbiology and Institute for Cellular and Molecular Biology, University of Texas, Austin, TX 78712. Phone: (512) 471-3817. Fax: (512) 471-7088. E-mail: rmeyer{at}mail.utexas.edu.

Journal of Bacteriology, May 1999, p. 2973-2978, Vol. 181, No. 9
0021-9193/99/$04.00+0
Copyright © 1999, American Society for Microbiology. All rights reserved.


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