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Journal of Bacteriology, January 2000, p. 225-227, Vol. 182, No. 1
0021-9193/0/$04.00+0
Copyright © 2000, American Society for Microbiology. All rights reserved.
Inorganic Polyphosphate Is Required for Motility of
Bacterial Pathogens
M. Harunur
Rashid,
Narayana
N.
Rao, and
Arthur
Kornberg*
Department of Biochemistry, Stanford
University School of Medicine, Stanford, California 94305
Received 19 April 1999/Accepted 11 October 1999
The ppk gene encodes polyphosphate kinase (PPK), the
principal enzyme in many bacteria responsible for the synthesis of
inorganic polyphosphate (polyP) from ATP. A null mutation in the
ppk gene of six bacterial pathogens renders them greatly
impaired in motility on semisolid agar plates; this defect can be
corrected by the introduction of ppk gene in
trans. In view of the fact that the motility of pathogens
is essential to invade and establish systemic infections in host cells,
this impairment in motility suggests a crucial and essential role of
PPK or polyP in bacterial pathogenesis.
*
Corresponding author. Mailing address: Department of
Biochemistry, Stanford University School of Medicine, Stanford, CA
94305. Phone: (650) 723-6167. Fax: (650) 723-6783. E-mail:
akornber{at}cmgm.stanford.edu.
Journal of Bacteriology, January 2000, p. 225-227, Vol. 182, No. 1
0021-9193/0/$04.00+0
Copyright © 2000, American Society for Microbiology. All rights reserved.
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