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Journal of Bacteriology, January 2000, p. 248-251, Vol. 182, No. 1
0021-9193/0/$04.00+0
Copyright © 2000, American Society for Microbiology. All rights reserved.

Elongation Factor Tu and DnaK Are Transferred from the Cytoplasm to the Periplasm of Escherichia coli during Osmotic Downshock Presumably via the Mechanosensitive Channel MscL

Catherine Berrier,1 Alexia Garrigues,1,dagger Gilbert Richarme,2 and Alexandre Ghazi1,*

Laboratoire des Biomembranes, UMR CNRS 8619, Université Paris-Sud, 91405 Orsay Cedex,1 and Biochimie Génétique, Institut Jacques Monod, Université Paris 7, Paris Cedex 05, 75251,2 France

Received 13 April 1999/Accepted 5 October 1999

Upon osmotic downshock, a few cytoplasmic proteins, including thioredoxin, elongation factor Tu (EF-Tu), and DnaK, are released from Tris-EDTA-treated Escherichia coli cells by an unknown mechanism. We have shown previously that deletion of mscL, the gene coding for the mechanosensitive channel of the plasma membrane with the highest conductance, prevents the release of thioredoxin. We confirm and extend the implication of MscL in this process by showing that the release of EF-Tu and DnaK is severely impaired in MscL-deficient strains. Release of these proteins is not observed in the absence of a Tris-EDTA treatment which disrupts the outer membrane, indicating that, in intact cells, they are transferred to the periplasm upon shock, presumably through the MscL channel.

* Corresponding author. Mailing address: Laboratoire des Biomembranes, UMR CNRS 8619, Bât. 430, Université Paris-Sud, 91405 Orsay Cedex, France. Phone: 33 1 69 15 71 94. Fax: 33 1 69 85 37 15. E-mail: alexandre.ghazi{at}biomemb.u-psud.fr.

dagger Present address: Section de Biophysique des Protéines et des Membranes, DBCM, CEA, Centre de Saclay, 91191 Gif sur Yvette Cedex, France.

Journal of Bacteriology, January 2000, p. 248-251, Vol. 182, No. 1
0021-9193/0/$04.00+0
Copyright © 2000, American Society for Microbiology. All rights reserved.


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