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Journal of Bacteriology, January 2000, p. 76-80, Vol. 182, No. 1
0021-9193/0/$04.00+0
Copyright © 2000, American Society for Microbiology. All rights reserved.

Copper/Zinc-Superoxide Dismutase Is Required for Oxytetracycline Resistance of Saccharomyces cerevisiae

Simon V. Avery,* Srividya Malkapuram,dagger Carolina Mateus, and Kimberly S. BabbDagger

Department of Biology, Georgia State University, Atlanta, Georgia 30303

Received 5 August 1999/Accepted 5 October 1999

Saccharomyces cerevisiae, along with other eukaryotes, is resistant to tetracyclines. We found that deletion of SOD1 (encoding Cu/Zn superoxide dismutase) rendered S. cerevisiae hypersensitive to oxytetracycline (OTC): a sod1Delta mutant exhibited a >95% reduction in colony-forming ability at an OTC concentration of 20 µg ml-1, whereas concentrations of up to 1,000 µg ml-1 had no effect on the growth of the wild type. OTC resistance was restored in the sod1Delta mutant by complementation with wild-type SOD1. The effect of OTC appeared to be cytotoxic and was not evident in a ctt1Delta (cytosolic catalase) mutant or in the presence of tetracycline. SOD1 transcription was not induced by OTC, suggesting that constitutive SOD1 expression is sufficient for wild-type OTC resistance. OTC uptake levels in wild-type and sod1Delta strains were similar. However, lipid peroxidation and protein oxidation were both enhanced during exposure of the sod1Delta mutant, but not the wild type, to OTC. We propose that Sod1p protects S. cerevisiae against a mode of OTC action that is dependent on oxidative damage.

* Corresponding author. Mailing address: Department of Biology, Georgia State University, University Plaza, Atlanta, GA 30303. Phone: (404) 651-0912. Fax: (404) 651-2509. E-mail: biosva{at}panther.gsu.edu.

dagger Present address: Emory University School of Medicine, Atlanta, GA 30322.

Dagger Present address: Boston University School of Medicine, Boston, MA.

Journal of Bacteriology, January 2000, p. 76-80, Vol. 182, No. 1
0021-9193/0/$04.00+0
Copyright © 2000, American Society for Microbiology. All rights reserved.


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