This Article
Right arrow Full Text
Right arrow Full Text (PDF)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrowReprints and Permissions
Right arrow Copyright Information
Right arrow Books from ASM Press
Right arrow MicrobeWorld
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Krause, S.
Right arrow Articles by Lanka, E.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Krause, S.
Right arrow Articles by Lanka, E.

 Previous Article  |  Next Article 

Journal of Bacteriology, May 2000, p. 2761-2770, Vol. 182, No. 10
0021-9193/00/$04.00+0
Copyright © 2000, American Society for Microbiology. All rights reserved.

Enzymology of Type IV Macromolecule Secretion Systems: the Conjugative Transfer Regions of Plasmids RP4 and R388 and the cag Pathogenicity Island of Helicobacter pylori Encode Structurally and Functionally Related Nucleoside Triphosphate Hydrolases

Sabine Krause,1 Werner Pansegrau,2,dagger Rudi Lurz,1 Fernando de la Cruz,3 and Erich Lanka1,*

Max-Planck-Institut für Molekulare Genetik, D-14195 Berlin, Germany1; Institute for Molecular Plant Sciences, Clusius Laboratory, Leiden University, 2333 AL Leiden, The Netherlands2; and Departamento de Biologia Molecular, Facultad de Medicina, Universidad de Cantabria, s/n 39011 Santander, Spain3

Received 13 December 1999/Accepted 1 March 2000

Type IV secretion systems direct transport of protein or nucleoprotein complexes across the cell envelopes of prokaryotic donor and eukaryotic or prokaryotic recipient cells. The process is mediated by a membrane-spanning multiprotein assembly. Potential NTPases belonging to the VirB11 family are an essential part of the membrane-spanning complex. Three representatives of these NTPases originating from the conjugative transfer regions of plasmids RP4 (TrbB) and R388 (TrwD) and from the cag pathogenicity island of Helicobacter pylori (HP0525) were overproduced and purified in native form. The proteins display NTPase activity with distinct substrate specificities in vitro. TrbB shows its highest specific hydrolase activity with dATP, and the preferred substrate for HP0525 is ATP. Analysis of defined TrbB mutations altered in motifs conserved within the VirB11 protein family shows that there is a correlation between the loss or reduction of NTPase activity and transfer frequency. Tryptophan fluorescence spectroscopy of TrbB and HP0525 suggests that both interact with phospholipid membranes, changing their conformation. NTPase activity of both proteins was stimulated by the addition of certain phospholipids. According to our results, Virb11-like proteins seem to most likely be involved in the assembly of the membrane-spanning multiprotein complex.

* Corresponding author. Mailing address: Max-Planck-Institut für Molekulare Genetik, Abteilung Lehrach, Ihnestrasse 73, D-14195 Berlin, Germany. Phone: 49 30 8413 1696. Fax: 49 30 8413 1130. E-mail: lanka{at}molgen.mpg.de.

dagger Present address: IRIS Research Center, Chiron S.p.A., I-53100 Siena, Italy.

Journal of Bacteriology, May 2000, p. 2761-2770, Vol. 182, No. 10
0021-9193/00/$04.00+0
Copyright © 2000, American Society for Microbiology. All rights reserved.


This article has been cited by other articles:

  • Kutter, S., Buhrdorf, R., Haas, J., Schneider-Brachert, W., Haas, R., Fischer, W. (2008). Protein Subassemblies of the Helicobacter pylori Cag Type IV Secretion System Revealed by Localization and Interaction Studies. J. Bacteriol. 190: 2161-2171 [Abstract] [Full Text]  
  • Hilleringmann, M., Pansegrau, W., Doyle, M., Kaufman, S., MacKichan, M. L., Gianfaldoni, C., Ruggiero, P., Covacci, A. (2006). Inhibitors of Helicobacter pylori ATPase Cag{alpha} block CagA transport and cag virulence.. Microbiology 152: 2919-2930 [Abstract] [Full Text]  
  • Chen, I., Christie, P. J., Dubnau, D. (2005). The Ins and Outs of DNA Transfer in Bacteria. Science 310: 1456-1460 [Abstract] [Full Text]  
  • Schoenhofen, I. C., Li, G., Strozen, T. G., Howard, S. P. (2005). Purification and Characterization of the N-Terminal Domain of ExeA: a Novel ATPase Involved in the Type II Secretion Pathway of Aeromonas hydrophila. J. Bacteriol. 187: 6370-6378 [Abstract] [Full Text]  
  • Crowther, L. J., Yamagata, A., Craig, L., Tainer, J. A., Donnenberg, M. S. (2005). The ATPase Activity of BfpD Is Greatly Enhanced by Zinc and Allosteric Interactions with Other Bfp Proteins. J. Biol. Chem. 280: 24839-24848 [Abstract] [Full Text]  
  • Chiang, P., Habash, M., Burrows, L. L. (2005). Disparate Subcellular Localization Patterns of Pseudomonas aeruginosa Type IV Pilus ATPases Involved in Twitching Motility. J. Bacteriol. 187: 829-839 [Abstract] [Full Text]  
  • Middleton, R., Sjolander, K., Krishnamurthy, N., Foley, J., Zambryski, P. (2005). Predicted hexameric structure of the Agrobacterium VirB4 C terminus suggests VirB4 acts as a docking site during type IV secretion. Proc. Natl. Acad. Sci. USA 102: 1685-1690 [Abstract] [Full Text]  
  • Camberg, J. L., Sandkvist, M. (2005). Molecular Analysis of the Vibrio cholerae Type II Secretion ATPase EpsE. J. Bacteriol. 187: 249-256 [Abstract] [Full Text]  
  • Cascales, E., Christie, P. J. (2004). Agrobacterium VirB10, an ATP energy sensor required for type IV secretion. Proc. Natl. Acad. Sci. USA 101: 17228-17233 [Abstract] [Full Text]  
  • Yeo, H.-J., Waksman, G. (2004). Unveiling Molecular Scaffolds of the Type IV Secretion System. J. Bacteriol. 186: 1919-1926 [Full Text]  
  • Sexton, J. A., Pinkner, J. S., Roth, R., Heuser, J. E., Hultgren, S. J., Vogel, J. P. (2004). The Legionella pneumophila PilT Homologue DotB Exhibits ATPase Activity That Is Critical for Intracellular Growth. J. Bacteriol. 186: 1658-1666 [Abstract] [Full Text]  
  • Llosa, M., Zunzunegui, S., de la Cruz, F. (2003). Conjugative coupling proteins interact with cognate and heterologous VirB10-like proteins while exhibiting specificity for cognate relaxosomes. Proc. Natl. Acad. Sci. USA 100: 10465-10470 [Abstract] [Full Text]  
  • Schroder, G., Lanka, E. (2003). TraG-Like Proteins of Type IV Secretion Systems: Functional Dissection of the Multiple Activities of TraG (RP4) and TrwB (R388). J. Bacteriol. 185: 4371-4381 [Abstract] [Full Text]  
  • Grohmann, E., Muth, G., Espinosa, M. (2003). Conjugative Plasmid Transfer in Gram-Positive Bacteria. Microbiol. Mol. Biol. Rev. 67: 277-301 [Abstract] [Full Text]  
  • Tomasini, M. L., Zanussi, S., Sozzi, M., Tedeschi, R., Basaglia, G., De Paoli, P. (2003). Heterogeneity of cag Genotypes in Helicobacterpylori Isolates from Human Biopsy Specimens. J. Clin. Microbiol. 41: 976-980 [Abstract] [Full Text]  
  • Rabel, C., Grahn, A. M., Lurz, R., Lanka, E. (2003). The VirB4 Family of Proposed Traffic Nucleoside Triphosphatases: Common Motifs in Plasmid RP4 TrbE Are Essential for Conjugation and Phage Adsorption. J. Bacteriol. 185: 1045-1058 [Abstract] [Full Text]  
  • Herdendorf, T. J., McCaslin, D. R., Forest, K. T. (2002). Aquifex aeolicus PilT, Homologue of a Surface Motility Protein, Is a Thermostable Oligomeric NTPase. J. Bacteriol. 184: 6465-6471 [Abstract] [Full Text]  
  • Ward, D. V., Draper, O., Zupan, J. R., Zambryski, P. C. (2002). Inaugural Article: Peptide linkage mapping of the Agrobacterium tumefaciens vir-encoded type IV secretion system reveals protein subassemblies. Proc. Natl. Acad. Sci. USA 99: 11493-11500 [Abstract] [Full Text]  
  • Tauch, A., Schneiker, S., Selbitschka, W., Puhler, A., van Overbeek, L. S., Smalla, K., Thomas, C. M., Bailey, M. J., Forney, L. J., Weightman, A., Ceglowski, P., Pembroke, T., Tietze, E., Schroder, G., Lanka, E., van Elsas, J. D. (2002). The complete nucleotide sequence and environmental distribution of the cryptic, conjugative, broad-host-range plasmid pIPO2 isolated from bacteria of the wheat rhizosphere. Microbiology 148: 1637-1653 [Abstract] [Full Text]  
  • Schroder, G., Krause, S., Zechner, E. L., Traxler, B., Yeo, H.-J., Lurz, R., Waksman, G., Lanka, E. (2002). TraG-Like Proteins of DNA Transfer Systems and of the Helicobacter pylori Type IV Secretion System: Inner Membrane Gate for Exported Substrates?. J. Bacteriol. 184: 2767-2779 [Abstract] [Full Text]  
  • Machon, C., Rivas, S., Albert, A., Goni, F. M., de la Cruz, F. (2002). TrwD, the Hexameric Traffic ATPase Encoded by Plasmid R388, Induces Membrane Destabilization and Hemifusion of Lipid Vesicles. J. Bacteriol. 184: 1661-1668 [Abstract] [Full Text]  
  • Cao, T. B., Saier, M. H. Jr (2001). Conjugal type IV macromolecular transfer systems of Gram-negative bacteria: organismal distribution, structural constraints and evolutionary conclusions. Microbiology 147: 3201-3214 [Full Text]  
  • Sagulenko, E., Sagulenko, V., Chen, J., Christie, P. J. (2001). Role of Agrobacterium VirB11 ATPase in T-Pilus Assembly and Substrate Selection. J. Bacteriol. 183: 5813-5825 [Abstract] [Full Text]  
  • Bhattacharjee, M. K., Kachlany, S. C., Fine, D. H., Figurski, D. H. (2001). Nonspecific Adherence and Fibril Biogenesis by Actinobacillus actinomycetemcomitans: TadA Protein Is an ATPase. J. Bacteriol. 183: 5927-5936 [Abstract] [Full Text]  
  • Hamilton, H. L., Schwartz, K. J., Dillard, J. P. (2001). Insertion-Duplication Mutagenesis of Neisseria: Use in Characterization of DNA Transfer Genes in the Gonococcal Genetic Island. J. Bacteriol. 183: 4718-4726 [Abstract] [Full Text]  
  • Planet, P. J., Kachlany, S. C., DeSalle, R., Figurski, D. H. (2001). Phylogeny of genes for secretion NTPases: Identification of the widespread tadA subfamily and development of a diagnostic key for gene classification. Proc. Natl. Acad. Sci. USA 98: 2503-2508 [Abstract] [Full Text]  
  • Eisenbrandt, R., Kalkum, M., Lurz, R., Lanka, E. (2000). Maturation of IncP Pilin Precursors Resembles the Catalytic Dyad-Like Mechanism of Leader Peptidases. J. Bacteriol. 182: 6751-6761 [Abstract] [Full Text]  
  • Rashkova, S., Zhou, X.-R., Chen, J., Christie, P. J. (2000). Self-Assembly of the Agrobacterium tumefaciens VirB11 Traffic ATPase. J. Bacteriol. 182: 4137-4145 [Abstract] [Full Text]  
  • Sakai, D., Horiuchi, T., Komano, T. (2001). ATPase Activity and Multimer Formation of PilQ Protein Are Required for Thin Pilus Biogenesis in Plasmid R64. J. Biol. Chem. 276: 17968-17975 [Abstract] [Full Text]  


Copyright © 2009 by the American Society for Microbiology.   For an alternate route to Journals.ASM.org, visit: http://intl-journals.asm.org | More Info»