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Journal of Bacteriology, May 2000, p. 2802-2810, Vol. 182, No. 10
0021-9193/00/$04.00+0
Copyright © 2000, American Society for Microbiology. All rights reserved.

Characterization of an Extracellular Lipase Encoded by LIP2 in Yarrowia lipolytica

Georges Pignède,1 Huijie Wang,2 Franck Fudalej,1 Claude Gaillardin,2 Michel Seman,1 and Jean-Marc Nicaud2,*

Laboratoire Mayoly Spindler, Service Recherche, 78401 Chatou Cedex,1 and Laboratoire de Microbiologie et Génétique Moléculaire, INRA-CNRS, 78850 Thiverval-Grignon,2 France

Received 6 December 1999/Accepted 23 February 2000

We isolated the LIP2 gene from the lipolytic yeast Yarrowia lipolytica. It was found to encode a 334-amino-acid precursor protein. The secreted lipase is a 301-amino-acid glycosylated polypeptide which is a member of the triacylglycerol hydrolase family (EC 3.1.1.3). The Lip2p precursor protein is processed by the KEX2-like endoprotease encoded by XPR6. Deletion of the XPR6 gene resulted in the secretion of an active but less stable proenzyme. Thus, the pro region does not inhibit lipase secretion and activity. However, it does play an essential role in the production of a stable enzyme. Processing was found to be correct in LIP2A (multiple LIP2 copy integrant)-overexpressing strains, which secreted 100 times more activity than the wild type, demonstrating that XPR6 maturation was not limiting. No extracellular lipase activity was detected with the lip2 knockout (KO) strain, strongly suggesting that extracellular lipase activity results from expression of the LIP2 gene. Nevertheless, the lip2 KO strain is still able to grow on triglycerides, suggesting an alternative pathway for triglyceride utilization in Y. lipolytica.

* Corresponding author. Mailing address: Laboratoire de Microbiologie et Génétique Moléculaire, INRA Grignon-CNRS, BP 01, 78850 Thiverval-Grignon, France. Phone: 33-01-30-81-54-50. Fax: 33-01-30-81-54-57. E-mail: Nicaud{at}platon.grignon.inra.fr.

Journal of Bacteriology, May 2000, p. 2802-2810, Vol. 182, No. 10
0021-9193/00/$04.00+0
Copyright © 2000, American Society for Microbiology. All rights reserved.


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