This Article
Right arrow Full Text
Right arrow Full Text (PDF)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrowReprints and Permissions
Right arrow Copyright Information
Right arrow Books from ASM Press
Right arrow MicrobeWorld
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Shen, X.
Right arrow Articles by Dybvig, K.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Shen, X.
Right arrow Articles by Dybvig, K.

 Previous Article  |  Next Article 

Journal of Bacteriology, May 2000, p. 2900-2908, Vol. 182, No. 10
0021-9193/00/$04.00+0
Copyright © 2000, American Society for Microbiology. All rights reserved.

Gene Rearrangements in the vsa Locus of Mycoplasma pulmonis

Xuejun Shen, Juliann Gumulak, Huilan Yu, C. Todd French, Nianxiang Zou, and Kevin Dybvig*

Department of Comparative Medicine, University of Alabama at Birmingham, Birmingham, Alabama 35294-0019

Received 2 December 1999/Accepted 25 February 2000

The vsa genes of Mycoplasma pulmonis encode the V-1 lipoproteins. Most V-1 proteins contain repetitive domains and are thought to be involved in mycoplasma-host cell interactions. Previously, we have reported the isolation and characterization of six vsa genes comprising a 10-kb region of the genome of M. pulmonis strain KD735-15. In the current study, vsa-specific probes were used to clone several fragments from a genomic library of KD735-15 DNA and assemble a single 20-kb contig containing 11 vsa genes. The middle region of the vsa locus contains a large open reading frame (ORF) that is not a vsa gene and has undergone an internal deletion in some strains. The ORF is predicted to encode a membrane protein that may have a role in disease pathogenesis. To examine vsa genes in a strain of M. pulmonis that is unrelated to KD735-15, strain CT was studied. Through Southern hybridization and genomic cloning analyses, CT was found to possess homologs of the KD735-15 vsaA, -C, -E, and -F genes and two unique genes (vsaG and vsaH) that were not found in KD735-15. High-frequency, site-specific DNA inversions serve to regulate the phase-variable production of individual V-1 proteins. As a result of the sequence analysis of vsa recombination products, a model in which DNA inversion arises from strand exchange involving at least six nucleotides of the vrs box is proposed.

* Corresponding author. Mailing address: Department of Comparative Medicine, University of Alabama at Birmingham, Volker Hall, Room 418A, Birmingham, AL 35294-0019. Phone: (205) 934-9327. Fax: (205) 975-4418. E-mail: dybvig{at}uab.edu.

Journal of Bacteriology, May 2000, p. 2900-2908, Vol. 182, No. 10
0021-9193/00/$04.00+0
Copyright © 2000, American Society for Microbiology. All rights reserved.


This article has been cited by other articles:

  • Nouvel, L.-X., Marenda, M., Sirand-Pugnet, P., Sagne, E., Glew, M., Mangenot, S., Barbe, V., Barre, A., Claverol, S., Citti, C. (2009). Occurrence, Plasticity, and Evolution of the vpma Gene Family, a Genetic System Devoted to High-Frequency Surface Variation in Mycoplasma agalactiae. J. Bacteriol. 191: 4111-4121 [Abstract] [Full Text]  
  • Horino, A., Kenri, T., Sasaki, Y., Okamura, N., Sasaki, T. (2009). Identification of a site-specific tyrosine recombinase that mediates promoter inversions of phase-variable mpl lipoprotein genes in Mycoplasma penetrans. Microbiology 155: 1241-1249 [Abstract] [Full Text]  
  • Madsen, M. L., Oneal, M. J., Gardner, S. W., Strait, E. L., Nettleton, D., Thacker, E. L., Minion, F. C. (2007). Array-Based Genomic Comparative Hybridization Analysis of Field Strains of Mycoplasma hyopneumoniae. J. Bacteriol. 189: 7977-7982 [Abstract] [Full Text]  
  • Rajan, I., Aravamuthan, S., Mande, S. S. (2007). Identification of compositionally distinct regions in genomes using the centroid method. Bioinformatics 23: 2672-2677 [Abstract] [Full Text]  
  • Simmons, W. L., Dybvig, K. (2007). Biofilms Protect Mycoplasma pulmonis Cells from Lytic Effects of Complement and Gramicidin. Infect. Immun. 75: 3696-3699 [Abstract] [Full Text]  
  • Simmons, W. L., Bolland, J. R., Daubenspeck, J. M., Dybvig, K. (2007). A Stochastic Mechanism for Biofilm Formation by Mycoplasma pulmonis. J. Bacteriol. 189: 1905-1913 [Abstract] [Full Text]  
  • Gyohda, A., Zhu, S., Furuya, N., Komano, T. (2006). Asymmetry of Shufflon-specific Recombination Sites in Plasmid R64 Inhibits Recombination between Direct sfx Sequences. J. Biol. Chem. 281: 20772-20779 [Abstract] [Full Text]  
  • Bowden, M. G., Chen, W., Singvall, J., Xu, Y., Peacock, S. J., Valtulina, V., Speziale, P., Hook, M. (2005). Identification and preliminary characterization of cell-wall-anchored proteins of Staphylococcus epidermidis. Microbiology 151: 1453-1464 [Abstract] [Full Text]  
  • Denison, A. M., Clapper, B., Dybvig, K. (2005). Avoidance of the Host Immune System through Phase Variation in Mycoplasma pulmonis. Infect. Immun. 73: 2033-2039 [Abstract] [Full Text]  
  • Gaurivaud, P., Persson, A., Grand, D. L., Westberg, J., Solsona, M., Johansson, K.-E., Poumarat, F. (2004). Variability of a glucose phosphotransferase system permease in Mycoplasma mycoides subsp. mycoides Small Colony. Microbiology 150: 4009-4022 [Abstract] [Full Text]  
  • Jaffe, J. D., Stange-Thomann, N., Smith, C., DeCaprio, D., Fisher, S., Butler, J., Calvo, S., Elkins, T., FitzGerald, M. G., Hafez, N., Kodira, C. D., Major, J., Wang, S., Wilkinson, J., Nicol, R., Nusbaum, C., Birren, B., Berg, H. C., Church, G. M. (2004). The Complete Genome and Proteome of Mycoplasma mobile. Genome Res 14: 1447-1461 [Abstract] [Full Text]  
  • van der Woude, M. W., Baumler, A. J. (2004). Phase and Antigenic Variation in Bacteria. Clin. Microbiol. Rev. 17: 581-611 [Abstract] [Full Text]  
  • Simmons, W. L., Dybvig, K. (2003). The Vsa Proteins Modulate Susceptibility of Mycoplasma pulmonis to Complement Killing, Hemadsorption, and Adherence to Polystyrene. Infect. Immun. 71: 5733-5738 [Abstract] [Full Text]  
  • Flitman-Tene, R., Mudahi-Orenstein, S., Levisohn, S., Yogev, D. (2003). Variable Lipoprotein Genes of Mycoplasma agalactiae Are Activated In Vivo by Promoter Addition via Site-Specific DNA Inversions. Infect. Immun. 71: 3821-3830 [Abstract] [Full Text]  
  • Horino, A., Sasaki, Y., Sasaki, T., Kenri, T. (2003). Multiple Promoter Inversions Generate Surface Antigenic Variation in Mycoplasma penetrans. J. Bacteriol. 185: 231-242 [Abstract] [Full Text]  
  • Calcutt, M. J., Lewis, M. S., Wise, K. S. (2002). Molecular Genetic Analysis of ICEF, an Integrative Conjugal Element That Is Present as a Repetitive Sequence in the Chromosome of Mycoplasma fermentans PG18. J. Bacteriol. 184: 6929-6941 [Abstract] [Full Text]  
  • Glew, M. D., Marenda, M., Rosengarten, R., Citti, C. (2002). Surface Diversity in Mycoplasma agalactiae Is Driven by Site-Specific DNA Inversions within the vpma Multigene Locus. J. Bacteriol. 184: 5987-5998 [Abstract] [Full Text]  
  • Persson, A., Jacobsson, K., Frykberg, L., Johansson, K.-E., Poumarat, F. (2002). Variable Surface Protein Vmm of Mycoplasma mycoides subsp. mycoides Small Colony Type. J. Bacteriol. 184: 3712-3722 [Abstract] [Full Text]  
  • Rocha, E. P. C., Blanchard, A. (2002). Genomic repeats, genome plasticity and the dynamics of Mycoplasma evolution. Nucleic Acids Res 30: 2031-2042 [Abstract] [Full Text]  
  • Nussbaum, S., Lysnyansky, I., Sachse, K., Levisohn, S., Yogev, D. (2002). Extended Repertoire of Genes Encoding Variable Surface Lipoproteins in Mycoplasma bovis Strains. Infect. Immun. 70: 2220-2225 [Abstract] [Full Text]  
  • Teachman, A. M., French, C. T., Yu, H., Simmons, W. L., Dybvig, K. (2002). Gene Transfer in Mycoplasma pulmonis. J. Bacteriol. 184: 947-951 [Abstract] [Full Text]  
  • Lysnyansky, I., Ron, Y., Yogev, D. (2001). Juxtaposition of an Active Promoter to vsp Genes via Site-Specific DNA Inversions Generates Antigenic Variation in Mycoplasma bovis. J. Bacteriol. 183: 5698-5708 [Abstract] [Full Text]  
  • Chambaud, I., Heilig, R., Ferris, S., Barbe, V., Samson, D., Galisson, F., Moszer, I., Dybvig, K., Wroblewski, H., Viari, A., Rocha, E. P.C., Blanchard, A. (2001). The complete genome sequence of the murine respiratory pathogen Mycoplasma pulmonis. Nucleic Acids Res 29: 2145-2153 [Abstract] [Full Text]  


Copyright © 2009 by the American Society for Microbiology.   For an alternate route to Journals.ASM.org, visit: http://intl-journals.asm.org | More Info»