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Journal of Bacteriology, May 2000, p. 2909-2918, Vol. 182, No. 10
0021-9193/00/$04.00+0

Altered Stationary-Phase Response in a Borrelia burgdorferi rpoS Mutant

Abdallah F. Elias,1,* James L. Bono,1 James A. Carroll,2 Philip Stewart,1 Kit Tilly,1 and Patricia Rosa1

Laboratory of Human Bacterial Pathogenesis1 and Microscopy Branch,2 Rocky Mountain Laboratories, National Institute of Allergy and Infectious Diseases, National Institutes of Health, Hamilton, Montana 59840

Received 22 November 1999/Accepted 23 February 2000

The homolog of the chromosomally encoded stationary-phase sigma factor RpoS in Borrelia burgdorferi was inactivated using gyrBr as a selectable marker. Two-dimensional nonequilibrium pH gradient electrophoresis of stationary-phase cell lysates identified at least 11 differences between the protein profiles of the rpoS mutant and wild-type organisms. Wild-type B. burgdorferi had a growth phase-dependent resistance to 1 N NaCl, similar to the stationary-phase response reported for other bacteria. The B. burgdorferi rpoS mutant strain was less resistant to osmotic stress in stationary phase than the isogenic rpoS wild-type organism. The results indicate that the B. burgdorferi rpoS homolog influences protein composition and participates in stationary-phase-dependent osmotic resistance. This rpoS mutant will be useful for studying regulation of gene expression in response to changing environmental conditions.

* Corresponding author. Mailing address: Rocky Mountain Laboratories, Laboratory of Human Bacterial Pathogenesis, National Institute of Allergy and Infectious Diseases, National Institutes of Health, 903 South 4th Street, Hamilton, MT 59840. Phone: (406) 363-9301. Fax: (406) 363-9204. E-mail: aelias{at}niaid.nih.gov.

Journal of Bacteriology, May 2000, p. 2909-2918, Vol. 182, No. 10
0021-9193/00/$04.00+0


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