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Journal of Bacteriology, May 2000, p. 2937-2944, Vol. 182, No. 10
0021-9193/00/$04.00+0
Copyright © 2000, American Society for Microbiology. All rights reserved.

NhaA, an Na+/H+ Antiporter Involved in Environmental Survival of Vibrio cholerae

Sophie Vimont and Patrick Berche*

Institut National de la Santé et de la Recherche Médicale (INSERM U411), CHU Necker-Enfants-Malades, 75730 Paris Cedex 15, France

Received 1 November 1999/Accepted 29 February 2000

Vibrio cholerae, the agent of cholera, is a normal inhabitant of aquatic environments, in which it survives under a wide range of conditions of pH and salinity. In this work, we identified the nhaA gene in a wild-type epidemic strain of V. cholerae O1. nhaA encodes a protein of 382 amino acids that is very similar to the proteins NhaA of Vibrio parahaemolyticus, Vibrio alginolyticus (~87% identity), and Escherichia coli (56% identity). V. cholerae NhaA complements an E. coli nhaA mutant, enabling it to grow in 700 mM NaCl, pH 7.5, indicating functional homology to E. coli NhaA. However, unlike E. coli, the growth of a nhaA-inactivated mutant of V. cholerae was not restricted at various pH and NaCl concentrations, although it was inhibited in the presence of 120 mM LiCl at pH 8.5. Nevertheless, using a nhaA'-lacZ transcriptional fusion, we observed induction of nhaA transcription by Na+, Li+, and K+. These results strongly suggest that NhaA is an Na+/H+ antiporter contributing to the Na+/H+ homeostasis of V. cholerae. nhaA-related sequences were detected in all strains of V. cholerae from the various serogroups. This gene is presumably involved in the survival and persistence of free-living bacteria in their natural habitat.

* Corresponding author. Mailing address: INSERM U411, CHU Necker-Enfants-Malades, 156, rue de Vaugirard, 75730 Paris Cedex 15, France. Phone: (33) 1 40 61 53 73. Fax: (33) 1 40 61 55 92. E-mail: berche{at}necker.fr.

Journal of Bacteriology, May 2000, p. 2937-2944, Vol. 182, No. 10
0021-9193/00/$04.00+0
Copyright © 2000, American Society for Microbiology. All rights reserved.


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