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Journal of Bacteriology, May 2000, p. 2945-2952, Vol. 182, No. 10
0021-9193/00/$04.00+0
Copyright © 2000, American Society for Microbiology. All rights reserved.

Pressure Regulation of Soluble Cytochromes c in a Deep-Sea Piezophilic Bacterium, Shewanella violacea

Mitsunori Yamada,1,2,dagger Kaoru Nakasone,1 Hideyuki Tamegai,1,3 Chiaki Kato,1,* Ron Usami,2 and Koki Horikoshi1,2

The DEEPSTAR Group, Japan Marine Science and Technology Center, Yokosuka 237-0061,1 Department of Applied Chemistry, Faculty of Engineering, Toyo University, Kawagoe-shi, Saitama 350-0815,2 and Department of Chemistry, Faculty of Science, Tokyo Institute of Technology, Meguro-ku, Tokyo 152-8551,3 Japan

Received 25 October 1999/Accepted 28 February 2000

Two c-type cytochromes from the soluble fraction of a deep-sea moderately piezophilic bacterium, Shewanella violacea, were purified and characterized, and the genes coding for these cytochromes were cloned and sequenced. One of the cytochromes, designated cytochrome cA, was found to have a molecular mass of approximately 8.3 kDa, and it contained one heme c per molecule. The other, designated cytochrome cB, was found to have a molecular mass of approximately 23 kDa, and it contained two heme c molecules per protein molecule. The amount of cytochrome cB expressed in cells grown at high hydrostatic pressure (50 MPa) was less than that in cells grown at atmospheric pressure, whereas cytochrome cA was constitutively expressed under all pressure conditions examined. The results of Northern blotting analysis were consistent with the above-mentioned observations and suggested that the pressure regulation of cytochrome cB gene expression occurred at the transcriptional level. These results suggest that the components of the respiratory chain of moderately piezophilic S. violacea could be exchanged according to the growth pressure conditions.

* Corresponding author. Mailing address: The DEEPSTAR Group, Japan Marine Science and Technology Center, 2-15 Natsushima-cho, Yokosuka 237-0061, Japan. Phone: 81-468-67-5555. Fax: 81-468-66-6364. E-mail: katoc{at}jamstec.go.jp.

dagger Present address: Department of BioScience, Faculty of Science, Tokyo Metropolitan University, Hachiouji, Tokyo 192-0316, Japan.

Journal of Bacteriology, May 2000, p. 2945-2952, Vol. 182, No. 10
0021-9193/00/$04.00+0
Copyright © 2000, American Society for Microbiology. All rights reserved.


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