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Journal of Bacteriology, May 2000, p. 2960-2966, Vol. 182, No. 10
0021-9193/00/$04.00+0
Copyright © 2000, American Society for Microbiology. All rights reserved.

The ArcB Sensor Kinase of Escherichia coli: Genetic Exploration of the Transmembrane Region

Ohsuk Kwon,1 Dimitris Georgellis,1 A. Simon Lynch,2 Dana Boyd,1 and E. C. C. Lin1,*

Department of Microbiology and Molecular Genetics, Harvard Medical School, Boston, Massachusetts 02115,1 and Bacterial Diseases Group, Tularik Inc., South San Francisco, California 940802

Received 10 January 2000/Accepted 3 March 2000

The Arc two-component signal transduction system of Escherichia coli regulates the expression of numerous operons in response to respiratory growth conditions. Cellular redox state or proton motive force (Delta <A><AC>&mgr;</AC><AC>¯</AC></A>H+) has been proposed to be the signal for the membrane-associated ArcB sensor kinase. This study provided evidence for a short ArcB periplasmic bridge that contains a His47. The dispensability of this amino acid, the only amino acid with a pK in the physiological range, renders the Delta <A><AC>&mgr;</AC><AC>¯</AC></A>H+ model unlikely. Furthermore, results from substituting membrane segments of ArcB with counterparts of MalF indicate that the region does not play a stereospecific role in signal reception.

* Corresponding author. Mailing address: Department of Microbiology and Molecular Genetics, Harvard Medical School, 200 Longwood Ave., Boston, MA 02115. Phone: (617) 432-1925. Fax: (617) 738-7664. E-mail: elin{at}hms.harvard.edu.

Journal of Bacteriology, May 2000, p. 2960-2966, Vol. 182, No. 10
0021-9193/00/$04.00+0
Copyright © 2000, American Society for Microbiology. All rights reserved.


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