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Journal of Bacteriology, May 2000, p. 2960-2966, Vol. 182, No. 10
0021-9193/00/$04.00+0
Copyright © 2000, American Society for Microbiology. All rights reserved.
The ArcB Sensor Kinase of Escherichia
coli: Genetic Exploration of the Transmembrane Region
Ohsuk
Kwon,1
Dimitris
Georgellis,1
A. Simon
Lynch,2
Dana
Boyd,1 and
E. C. C.
Lin1,*
Department of Microbiology and Molecular
Genetics, Harvard Medical School, Boston, Massachusetts
02115,1 and Bacterial Diseases
Group, Tularik Inc., South San Francisco, California
940802
Received 10 January 2000/Accepted 3 March 2000
The Arc two-component signal transduction system of
Escherichia coli regulates the expression of numerous
operons in response to respiratory growth conditions. Cellular redox
state or proton motive force (
H+) has been
proposed to be the signal for the membrane-associated ArcB sensor
kinase. This study provided evidence for a short ArcB periplasmic
bridge that contains a His47. The dispensability of this amino acid,
the only amino acid with a pK in the physiological range, renders the

H+ model unlikely. Furthermore, results
from substituting membrane segments of ArcB with counterparts of MalF
indicate that the region does not play a stereospecific role in signal reception.
*
Corresponding author. Mailing address: Department of
Microbiology and Molecular Genetics, Harvard Medical School, 200 Longwood Ave., Boston, MA 02115. Phone: (617) 432-1925. Fax: (617)
738-7664. E-mail: elin{at}hms.harvard.edu.
Journal of Bacteriology, May 2000, p. 2960-2966, Vol. 182, No. 10
0021-9193/00/$04.00+0
Copyright © 2000, American Society for Microbiology. All rights reserved.
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