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Journal of Bacteriology, May 2000, p. 2973-2977, Vol. 182, No. 10
0021-9193/00/$04.00+0
Copyright © 2000, American Society for Microbiology. All rights reserved.

A Study of the CopF Repressor of Plasmid pAMbeta 1 by Phage Display

Emmanuelle d'Alençon and S. Dusko Ehrlich*

Génétique Microbienne, INRA, Domaine de Vilvert, 78352 Jouy en Josas Cedex, France

Received 20 October 1999/Accepted 28 February 2000

We studied DNA binding of a transcriptional repressor, CopF, displayed on a filamentous phage. Mutagenesis of a putative helix-turn-helix motif of CopF and of certain bases of the operator abolished the protein-DNA interaction, establishing the elements involved in CopF function and showing that phage display can be used to study repressor proteins.

* Corresponding author. Mailing address: Génétique Microbienne, INRA, Domaine de Vilvert, 78352 Jouy en Josas Cedex, France. Phone: 33 1 34 65 25 11. Fax: 31 1 34 65 25 21. E-mail: ehrlich{at}biotec.jouy.inra.fr.

Journal of Bacteriology, May 2000, p. 2973-2977, Vol. 182, No. 10
0021-9193/00/$04.00+0
Copyright © 2000, American Society for Microbiology. All rights reserved.


This article has been cited by other articles:

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  • Bulyk, M. L., Huang, X., Choo, Y., Church, G. M. (2001). Exploring the DNA-binding specificities of zinc fingers with DNA microarrays. Proc. Natl. Acad. Sci. USA 98: 7158-7163 [Abstract] [Full Text]  


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