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Journal of Bacteriology, May 2000, p. 2982-2984, Vol. 182, No. 10
0021-9193/00/$04.00+0
Copyright © 2000, American Society for Microbiology. All rights reserved.

Positioning of Region 4 of the Escherichia coli RNA Polymerase sigma 70 Subunit by a Transcription Activator

Jonathan A. Bown,1 Annie Kolb,2 Claude F. Meares,3 Akira Ishihama,4 Stephen D. Minchin,1 and Stephen J. W. Busby1,*

School of Biosciences, The University of Birmingham, Edgbaston, Birmingham B15 2TT, United Kingdom1; Institut Pasteur, Unité de Physico-chimie des Macromolécules Biologiques, 75724 Paris Cedex 15, France2; Department of Chemistry, University of California, Davis, California 956163; and Department of Molecular Genetics, National Institute of Genetics, Mishima, Shizuoka 411, Japan4

Received 10 September 1999/Accepted 12 January 2000

A DNA cleavage reagent, specifically tethered to residue 581 of the Escherichia coli RNA polymerase sigma 70 subunit, has been used to investigate the location of sigma 70 region 4 in different complexes at the galp1 promoter and the effect of the cyclic AMP receptor protein. The positions of DNA cleavage by the reagent are not affected by the cyclic AMP receptor protein. We conclude that transcription activation at the galp1 promoter by the cyclic AMP receptor protein does not involve major conformation changes in or repositioning of sigma 70 region 4.

* Corresponding author. Mailing address: School of Biosciences, The University of Birmingham, Edgbaston, Birmingham B15 2TT, United Kingdom. Phone: 44-121-414-5439. Fax: 44-121-414-7366. E-mail: S.J.W.Busby{at}bham.ac.uk.

Journal of Bacteriology, May 2000, p. 2982-2984, Vol. 182, No. 10
0021-9193/00/$04.00+0
Copyright © 2000, American Society for Microbiology. All rights reserved.


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