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Journal of Bacteriology, June 2000, p. 3281-3284, Vol. 182, No. 11
Zentrum für Molekulare Biologie
Heidelberg, Mikrobiologie Universität Heidelberg, 69120 Heidelberg, Germany
Received 12 November 1999/Accepted 15 March 2000
A highly abundant and heterogenous small RNA about 205 to 210 bases
long named MP200 RNA has been identified in Mycoplasma pneumoniae. It was localized on the genome within a 319-bp-long intergenic space of the pyruvate dehydrogenase (pdh) gene
cluster. A database search at the DNA level revealed the highest
similarity to a sequence located within the pdh gene
cluster of Mycoplasma genitalium that was also shown to be
transcribed into two abundant, but smaller RNAs than the ones in
Mycoplasma pneumoniae. The RNAs from both M. pneumoniae and M. genitalium have the potential to code for cysteine-rich 29- and 23-amino-acid-long peptides, but so far,
these peptides have not been identified experimentally in bacterial
protein extracts.
0021-9193/00/$04.00+0
Copyright © 2000, American Society for Microbiology. All rights reserved.
Identification of a Small RNA within the
pdh Gene Cluster of Mycoplasma pneumoniae and
Mycoplasma genitalium
and
*
Corresponding author. Mailing address: Zentrum
für Molekulare Biologie Heidelberg, Mikrobiologie
Universität Heidelberg, 69120 Heidelberg, Germany. Phone:
(49)-6221-54 68 27. Fax: (49)-6221-54 58 93. E-mail:
r.herrmann{at}mail.zmbh.uni-heidelberg.de.
Present address: Institut für Biochemie, Bayerische
Julius-Maximilians-Universität, Biozentrum, Würzburg, Germany.
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