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Journal of Bacteriology, June 2000, p. 3292-3297, Vol. 182, No. 11
0021-9193/00/$04.00+0
Copyright © 2000, American Society for Microbiology. All rights reserved.

Organization, Structure, and Variability of the rRNA Operon of the Whipple's Disease Bacterium (Tropheryma whippelii)

Matthias Maiwald,1 Axel von Herbay,2 Paul W. Lepp,1 and David A. Relman1,3,4,*

Departments of Microbiology and Immunology1 and Medicine,3 Stanford University School of Medicine, Stanford, California 94305; Veterans Affairs Palo Alto Health Care System, Palo Alto, California 943044; and Pathologisches Institut der Universität, 69120 Heidelberg, Germany2

Received 8 December 1999/Accepted 12 March 2000

Whipple's disease is a systemic disorder associated with a cultivation-resistant, poorly characterized actinomycete, Tropheryma whippelii. We determined a nearly complete rRNA operon sequence of T. whippelii from specimens from 3 patients with Whipple's disease, as well as partial operon sequences from 43 patients. Variability was observed in the 16S-23S rRNA spacer sequences, leading to the description of five distinct sequence types. One specimen contained two spacer sequence types, raising the possibility of a double infection. Secondary structure models for the primary rRNA transcript and mature rRNAs revealed rare or unique features.

* Corresponding author. Mailing address: VA Palo Alto Health Care System, 154T, 3801 Miranda Ave., Palo Alto, CA 94304. Phone: (650) 852-3308. Fax: (650) 852-3291. E-mail: relman{at}cmgm.stanford.edu.

Journal of Bacteriology, June 2000, p. 3292-3297, Vol. 182, No. 11
0021-9193/00/$04.00+0
Copyright © 2000, American Society for Microbiology. All rights reserved.


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