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Journal of Bacteriology, June 2000, p. 3302-3304, Vol. 182, No. 11
0021-9193/00/$04.00+0
Copyright © 2000, American Society for Microbiology. All rights reserved.

Characterization of the relA Gene of Porphyromonas gingivalis

Keya Sen,1 Jun-ichiro Hayashi,1 and Howard K. Kuramitsu1,2,*

Departments of Oral Biology1 and Microbiology,2 State University of New York, Buffalo, New York 14214

Received 20 September 1999/Accepted 7 March 2000

Based upon the nucleotide sequence of the relA gene from Escherichia coli, a gene fragment corresponding to the homologous gene from the pathogenic oral bacterium Porphyromonas gingivalis 381 was isolated by PCR and utilized to construct a relA mutant. The mutant, KS7, was defective in ribosome-mediated ppGpp formation and also in the stringent response.

* Corresponding author. Mailing address: Department of Oral Biology, SUNY, 3435 Main Street, Buffalo, NY 14214. Phone: (716) 829-2068. Fax: (716) 829-3942. E-mail: KURAMITS{at}ACSU.BUFFALO.EDU.

Journal of Bacteriology, June 2000, p. 3302-3304, Vol. 182, No. 11
0021-9193/00/$04.00+0
Copyright © 2000, American Society for Microbiology. All rights reserved.


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