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Journal of Bacteriology, June 2000, p. 3310-3313, Vol. 182, No. 11
0021-9193/00/$04.00+0
Copyright © 2000, American Society for Microbiology. All rights reserved.

Target Site Selection by Tn7: attTn7 Transcription and Target Activity

Robert T. DeBoydagger and Nancy L. Craig*

The Howard Hughes Medical Institute, Department of Molecular Biology and Genetics, The Johns Hopkins University School of Medicine, Baltimore, Maryland 21205

Received 29 October 1999/Accepted 14 March 2000

The bacterial transposon Tn7 inserts at high frequency into a specific site called attTn7, which is present in the chromosomes of many bacteria. We show here that transcription of a nearby gene, glmS, decreases the frequency of Tn7 insertion into attTn7, thus providing a link between Tn7 transposition and host cell metabolism.

* Corresponding author. Mailing address: The Howard Hughes Medical Institute, Department of Molecular Biology and Genetics, The Johns Hopkins University School of Medicine, 725 N. Wolfe St., 601 PCTB, Baltimore, MD 21205. Phone: (410) 955-3933. Fax: (410) 955-0831. E-mail: ncraig{at}jhmi.edu.

dagger Present address: The Institute for Genomic Research, Rockville, MD 20850.

Journal of Bacteriology, June 2000, p. 3310-3313, Vol. 182, No. 11
0021-9193/00/$04.00+0
Copyright © 2000, American Society for Microbiology. All rights reserved.


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