Differential Expression of over 60 Chromosomal Genes in Escherichia coli by Constitutive Expression of MarA

doi:10.1128/JB.182.12.3467-3474.2000

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Journal of Bacteriology, June 2000, p. 3467-3474, Vol. 182, No. 12
0021-9193/00/$04.00+0
Copyright © 2000, American Society for Microbiology. All rights reserved.

Differential Expression of over 60 Chromosomal Genes in Escherichia coli by Constitutive Expression of MarA

Teresa M. Barbosa¹ and Stuart B. Levy¹^,²^,^*

Center for Adaptation Genetics and Drug Resistance and the Departments of Molecular Biology and Microbiology¹ and Medicine,² Tufts University School of Medicine, Boston, Massachusetts 02111

Received 28 December 1999/Accepted 31 March 2000

In Escherichia coli, the MarA protein controls expression of multiple chromosomal genes affecting resistance to antibioticsand other environmental hazards. For a more-complete characterizationof the mar regulon, duplicate macroarrays containing 4,290 openreading frames of the E. coli genome were hybridized to radiolabeledcDNA populations derived from mar-deleted and mar-expressing E.coli. Strains constitutively expressing MarA showed altered expressionof more than 60 chromosomal genes: 76% showed increased expressionand 24% showed decreased expression. Although some of the geneswere already known to be MarA regulated, the majority were newlydetermined and belonged to a variety of functional groups. Someof the genes identified have been associated with iron transportand metabolism; other genes were previously known to be part ofthe soxRS regulon. Northern blot analysis of selected genes confirmedthe results obtained with the macroarrays. The findings revealthat the mar locus mediates a global stress response involvingone of the largest networks of genesdescribed.

^* Corresponding author. Mailing address: Center for Adaptation Genetics and Drug Resistance, Tufts University School of Medicine,136 Harrison Ave., Boston, MA 02111. Phone: (617) 636-6764. Fax:(617) 636-0458. E-mail: slevy{at}opal.tufts.edu.

Journal of Bacteriology, June 2000, p. 3467-3474, Vol. 182, No. 12
0021-9193/00/$04.00+0
Copyright © 2000, American Society for Microbiology. All rights reserved.

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