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Journal of Bacteriology, June 2000, p. 3587-3589, Vol. 182, No. 12
0021-9193/00/$04.00+0
Copyright © 2000, American Society for Microbiology. All rights reserved.

The Length of the Combined 3' Untranslated Region and Poly(A) Tail Does Not Control Rates of Glyceraldehyde-3-Phosphate Dehydrogenase mRNA Translation in Three Species of Parasitic Protists

Benno H. ter Kuile1,* and Fernando J. Sallés2

The Rockefeller University, New York, New York 10021-6399,1 and Department of Pharmacology, State University of New York at Stony Brook, Stony Brook, New York 11794-86512

Received 11 October 1999/Accepted 23 March 2000

Experimental observations suggested that the length of the glyceraldehyde-3-phosphate dehydrogenase (GAPDH) mRNA 3' end has a role in regulating rates of translation in the parasitic protists Trypanosoma brucei, Leishmania donovani, and Trichomonas vaginalis. Using a PCR assay for poly(A) tail length, we measured the size of the RNA 3' end under different growth conditions in all three species. Our results showed that the combined 3' untranslated region and poly(A) tail of GAPDH mRNA do not vary with different rates of translation.

* Corresponding author. Present address: Leiden Institute of Chemistry, Gorlaeus Laboratories, Leiden University, Einsteinweg 5/P.O. Box 9502, 2300 RA Leiden, The Netherlands. Phone: 31-71-5275272. Fax: 31-71-5274537. E-mail: b.terkuile{at}chem.leidenuniv.nl.

Journal of Bacteriology, June 2000, p. 3587-3589, Vol. 182, No. 12
0021-9193/00/$04.00+0
Copyright © 2000, American Society for Microbiology. All rights reserved.




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