This Article
Right arrow Full Text
Right arrow Full Text (PDF)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrowReprints and Permissions
Right arrow Copyright Information
Right arrow Books from ASM Press
Right arrow MicrobeWorld
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Joyce, E. A.
Right arrow Articles by Wright, A.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Joyce, E. A.
Right arrow Articles by Wright, A.

 Previous Article  |  Next Article 

Journal of Bacteriology, July 2000, p. 3638-3643, Vol. 182, No. 13
0021-9193/00/$04.00+0
Copyright © 2000, American Society for Microbiology. All rights reserved.

Evidence for a Signaling System in Helicobacter pylori: Detection of a luxS-Encoded Autoinducer

Elizabeth A. Joyce,1 Bonnie L. Bassler,2 and Andrew Wright1,*

Department of Microbiology and Molecular Biology, Tufts University School of Medicine, Boston, Massachusetts 02111,1 and Department of Molecular Biology, Princeton University, Princeton, New Jersey 08544-10142

Received 27 January 2000/Accepted 30 March 2000

Helicobacter pylori possesses a homolog of the luxS gene, initially identified by its role in autoinducer production for the quorum-sensing system 2 in Vibrio harveyi. The genomes of several other species of bacteria, notably Escherichia coli, Salmonella enterica serovar Typhimurium, and Vibrio cholerae, also include luxS homologs. All of these bacteria have been shown to produce active autoinducers capable of stimulating the expression of the luciferase operon in V. harveyi. In this report, we demonstrate that H. pylori also synthesizes a functional autoinducer (AI-2) that can specifically activate signaling system 2 in V. harveyi. Maximal activity is produced during early log phase, and the activity is diminished when cells enter stationary phase. We show that AI-2 is not involved in modulating any of the known or putative virulence factors in H. pylori and that a luxS null mutant has a two-dimensional protein profile identical to that of its isogenic parent strain. We discuss the implications of having an AI-2-like quorum-sensing system in H. pylori and suggest possible roles that it may play in H. pylori infection.

* Corresponding author. Mailing address: Department of Microbiology and Molecular Biology, Tufts University School of Medicine, 136 Harrison Ave., Boston, Massachusetts 02111. Phone: (617) 636-6760. Fax: (617) 636-0337. E-mail: awrigh02{at}granite.tufts.edu.

Journal of Bacteriology, July 2000, p. 3638-3643, Vol. 182, No. 13
0021-9193/00/$04.00+0
Copyright © 2000, American Society for Microbiology. All rights reserved.


This article has been cited by other articles:

  • Smith, T. G., Pereira, L., Hoover, T. R. (2009). Helicobacter pylori FlhB processing-deficient variants affect flagellar assembly but not flagellar gene expression. Microbiology 155: 1170-1180 [Abstract] [Full Text]  
  • Rader, B. A., Campagna, S. R., Semmelhack, M. F., Bassler, B. L., Guillemin, K. (2007). The Quorum-Sensing Molecule Autoinducer 2 Regulates Motility and Flagellar Morphogenesis in Helicobacter pylori. J. Bacteriol. 189: 6109-6117 [Abstract] [Full Text]  
  • Osaki, T., Hanawa, T., Manzoku, T., Fukuda, M., Kawakami, H., Suzuki, H., Yamaguchi, H., Yan, X., Taguchi, H., Kurata, S., Kamiya, S. (2006). Mutation of luxS affects motility and infectivity of Helicobacter pylori in gastric mucosa of a Mongolian gerbil model.. J Med Microbiol 55: 1477-1485 [Abstract] [Full Text]  
  • Lee, W.-K., Ogura, K., Loh, J. T., Cover, T. L., Berg, D. E. (2006). Quantitative Effect of luxS Gene Inactivation on the Fitness of Helicobacter pylori. Appl. Environ. Microbiol. 72: 6615-6622 [Abstract] [Full Text]  
  • Ndagijimana, M., Vallicelli, M., Cocconcelli, P. S., Cappa, F., Patrignani, F., Lanciotti, R., Guerzoni, M. E. (2006). Two 2[5H]-Furanones as Possible Signaling Molecules in Lactobacillus helveticus. Appl. Environ. Microbiol. 72: 6053-6061 [Abstract] [Full Text]  
  • Loh, J. T., Forsyth, M. H., Cover, T. L. (2004). Growth Phase Regulation of flaA Expression in Helicobacter pylori Is luxS Dependent. Infect. Immun. 72: 5506-5510 [Abstract] [Full Text]  
  • Winans, S. C. (2004). Reciprocal Regulation of Bioluminescence and Type III Protein Secretion in Vibrio harveyi and Vibrio parahaemolyticus in Response to Diffusible Chemical Signals. J. Bacteriol. 186: 3674-3676 [Full Text]  
  • Lupp, C., Ruby, E. G. (2004). Vibrio fischeri LuxS and AinS: Comparative Study of Two Signal Synthases. J. Bacteriol. 186: 3873-3881 [Abstract] [Full Text]  
  • Coulthurst, S. J., Kurz, C. L., Salmond, G. P. C. (2004). luxS mutants of Serratia defective in autoinducer-2-dependent 'quorum sensing' show strain-dependent impacts on virulence and production of carbapenem and prodigiosin. Microbiology 150: 1901-1910 [Abstract] [Full Text]  
  • Cole, S. P., Harwood, J., Lee, R., She, R., Guiney, D. G. (2004). Characterization of Monospecies Biofilm Formation by Helicobacter pylori. J. Bacteriol. 186: 3124-3132 [Abstract] [Full Text]  
  • Teplitski, M., Chen, H., Rajamani, S., Gao, M., Merighi, M., Sayre, R. T., Robinson, J. B., Rolfe, B. G., Bauer, W. D. (2004). Chlamydomonas reinhardtii Secretes Compounds That Mimic Bacterial Signals and Interfere with Quorum Sensing Regulation in Bacteria. Plant Physiol. 134: 137-146 [Abstract] [Full Text]  
  • Dove, J. E., Yasukawa, K., Tinsley, C. R., Nassif, X. (2003). Production of the signalling molecule, autoinducer-2, by Neisseria meningitidis: lack of evidence for a concerted transcriptional response. Microbiology 149: 1859-1869 [Abstract] [Full Text]  
  • Merrell, D. S., Goodrich, M. L., Otto, G., Tompkins, L. S., Falkow, S. (2003). pH-Regulated Gene Expression of the Gastric Pathogen Helicobacter pylori. Infect. Immun. 71: 3529-3539 [Abstract] [Full Text]  
  • Merritt, J., Qi, F., Goodman, S. D., Anderson, M. H., Shi, W. (2003). Mutation of luxS Affects Biofilm Formation in Streptococcus mutans. Infect. Immun. 71: 1972-1979 [Abstract] [Full Text]  
  • Hardie, K. R., Cooksley, C., Green, A. D., Winzer, K. (2003). Autoinducer 2 activity in Escherichia coli culture supernatants can be actively reduced despite maintenance of an active synthase, LuxS. Microbiology 149: 715-728 [Abstract] [Full Text]  
  • Mathesius, U., Mulders, S., Gao, M., Teplitski, M., Caetano-Anolles, G., Rolfe, B. G., Bauer, W. D. (2003). From the Cover: Extensive and specific responses of a eukaryote to bacterial quorum-sensing signals. Proc. Natl. Acad. Sci. USA 100: 1444-1449 [Abstract] [Full Text]  
  • McNab, R., Ford, S. K., El-Sabaeny, A., Barbieri, B., Cook, G. S., Lamont, R. J. (2003). LuxS-Based Signaling in Streptococcus gordonii: Autoinducer 2 Controls Carbohydrate Metabolism and Biofilm Formation with Porphyromonas gingivalis. J. Bacteriol. 185: 274-284 [Abstract] [Full Text]  
  • Derzelle, S., Duchaud, E., Kunst, F., Danchin, A., Bertin, P. (2002). Identification, Characterization, and Regulation of a Cluster of Genes Involved in Carbapenem Biosynthesis in Photorhabdus luminescens. Appl. Environ. Microbiol. 68: 3780-3789 [Abstract] [Full Text]  
  • Stevenson, B., Babb, K. (2002). LuxS-Mediated Quorum Sensing in Borrelia burgdorferi, the Lyme Disease Spirochete. Infect. Immun. 70: 4099-4105 [Abstract] [Full Text]  
  • Beeston, A. L., Surette, M. G. (2002). pfs-Dependent Regulation of Autoinducer 2 Production in Salmonella enterica Serovar Typhimurium. J. Bacteriol. 184: 3450-3456 [Abstract] [Full Text]  
  • Elvers, K. T., Park, S. F. (2002). Quorum sensing in Campylobacter jejuni: detection of a luxS encoded signalling molecule. Microbiology 148: 1475-1481 [Abstract] [Full Text]  
  • Winzer, K., Sun, Y.-h., Green, A., Delory, M., Blackley, D., Hardie, K. R., Baldwin, T. J., Tang, C. M. (2002). Role of Neisseria meningitidis luxS in Cell-to-Cell Signaling and Bacteremic Infection. Infect. Immun. 70: 2245-2248 [Abstract] [Full Text]  
  • Winzer, K., Hardie, K. R., Burgess, N., Doherty, N., Kirke, D., Holden, M. T. G., Linforth, R., Cornell, K. A., Taylor, A. J., Hill, P. J., Williams, P. (2002). LuxS: its role in central metabolism and the in vitro synthesis of 4-hydroxy-5-methyl-3(2H)-furanone. Microbiology 148: 909-922 [Abstract] [Full Text]  
  • Wen, Z. T., Burne, R. A. (2002). Functional Genomics Approach to Identifying Genes Required for Biofilm Development by Streptococcus mutans. Appl. Environ. Microbiol. 68: 1196-1203 [Abstract] [Full Text]  
  • Burgess, N. A., Kirke, D. F., Williams, P., Winzer, K., Hardie, K. R., Meyers, N. L., Aduse-Opoku, J., Curtis, M. A., Camara, M. (2002). LuxS-dependent quorum sensing in Porphyromonas gingivalis modulates protease and haemagglutinin activities but is not essential for virulence. Microbiology 148: 763-772 [Abstract] [Full Text]  
  • Schneider, R., Lockatell, C. V., Johnson, D., Belas, R. (2002). Detection and mutation of a luxS-encoded autoinducer in Proteus mirabilis. Microbiology 148: 773-782 [Abstract] [Full Text]  
  • Winans, S. C., Bassler, B. L. (2002). Mob Psychology. J. Bacteriol. 184: 873-883 [Full Text]  
  • Fong, K. P., Chung, W. O., Lamont, R. J., Demuth, D. R. (2001). Intra- and Interspecies Regulation of Gene Expression by Actinobacillus actinomycetemcomitans LuxS. Infect. Immun. 69: 7625-7634 [Abstract] [Full Text]  
  • Hilgers, M. T., Ludwig, M. L. (2001). Crystal structure of the quorum-sensing protein LuxS reveals a catalytic metal site. Proc. Natl. Acad. Sci. USA 10.1073/pnas.191223098v1 [Abstract] [Full Text]  
  • Rokbi, B., Seguin, D., Guy, B., Mazarin, V., Vidor, E., Mion, F., Cadoz, M., Quentin-Millet, M.-J. (2001). Assessment of Helicobacter pylori Gene Expression within Mouse and Human Gastric Mucosae by Real-Time Reverse Transcriptase PCR. Infect. Immun. 69: 4759-4766 [Abstract] [Full Text]  
  • Chung, W. O., Park, Y., Lamont, R. J., McNab, R., Barbieri, B., Demuth, D. R. (2001). Signaling System in Porphyromonas gingivalis Based on a LuxS Protein. J. Bacteriol. 183: 3903-3909 [Abstract] [Full Text]  
  • Schauder, S., Bassler, B. L. (2001). The languages of bacteria. Genes Dev. 15: 1468-1480 [Full Text]  
  • Hilgers, M. T., Ludwig, M. L. (2001). Crystal structure of the quorum-sensing protein LuxS reveals a catalytic metal site. Proc. Natl. Acad. Sci. USA 98: 11169-11174 [Abstract] [Full Text]  


Copyright © 2009 by the American Society for Microbiology.   For an alternate route to Journals.ASM.org, visit: http://intl-journals.asm.org | More Info»