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Journal of Bacteriology, July 2000, p. 3809-3815, Vol. 182, No. 13
0021-9193/00/$04.00+0
Copyright © 2000, American Society for Microbiology. All rights reserved.

Physical Morphology and Surface Properties of Unsaturated Pseudomonas putida Biofilms

Ilene D. Auerbach,1,2 Cody Sorensen,2 Helen G. Hansma,2 and Patricia A. Holden1,*

Donald Bren School of Environmental Science and Management1 and Department of Physics,2 University of California, Santa Barbara, California 93106

Received 10 January 2000/Accepted 2 April 2000

Unsaturated biofilms of Pseudomonas putida, i.e., biofilms grown in humid air, were analyzed by atomic force microscopy to determine surface morphology, roughness, and adhesion forces in the outer and basal cell layers of fresh and desiccated biofilms. Desiccated biofilms were equilibrated with a 75.5% relative humidity atmosphere, which is far below the relative humidity of 98 to 99% at which these biofilms were cultured. In sharp contrast to the effects of drying on biofilms grown in fluid, we observed that drying caused little change in morphology, roughness, or adhesion forces in these unsaturated biofilms. Surface roughness for moist and dry biofilms increased approximately linearly with increasing scan sizes. This indicated that the divides between bacteria contributed more to overall roughness than did extracellular polymeric substances (EPS) on individual bacteria. The EPS formed higher-order structures we termed mesostructures. These mesostructures are much larger than the discrete polymers of glycolipids and proteins that have been previously characterized on the outer surface of these gram-negative bacteria.

* Corresponding author. Mailing address: 4670 Physical Sciences Building North, University of California, Santa Barbara, CA 93106. Phone: (805) 893-3195. Fax: (805) 893-7612. E-mail: holden{at}bren.ucsb.edu.

Journal of Bacteriology, July 2000, p. 3809-3815, Vol. 182, No. 13
0021-9193/00/$04.00+0
Copyright © 2000, American Society for Microbiology. All rights reserved.


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