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Journal of Bacteriology, July 2000, p. 3920-3923, Vol. 182, No. 14
0021-9193/00/$04.00+0
Copyright © 2000, American Society for Microbiology. All rights reserved.

A Promoter Region Binding Protein and DNA Gyrase Regulate Anaerobic Transcription of nifLA in Enterobacter cloacae

Biao Hu, Jiabi Zhu, San Chiun Shen, and Guan-qiao Yu*

Laboratory of Molecular Genetics, Shanghai Institute of Plant Physiology, The Chinese Academy of Sciences, Shanghai, China 200032

Received 13 December 1999/Accepted 26 April 2000

Our work provides evidence that a sequence characteristic of FNR binding sites, when interacted with by a trans-acting factor, activates anaerobic transcription of the nifLA operon in Enterobacter cloacae. DNA gyrase activity has been found to be important for the anaerobic transcription of the nifLA promoter. Our results suggest that anaerobic regulation of the nifLA operon is mediated through the control of the promoter region-binding trans-acting factor at the transcriptional level, while DNA supercoiling functions in providing a topological requirement for the activation of transcription.

* Corresponding author. Mailing address: Laboratory of Molecular Genetics, Shanghai Institute of Plant Physiology, The Chinese Academy of Sciences, 300 Fenglin Rd., Shanghai, China 200032. Phone: 86-21-64042090-4319. Fax: 86-21-64042385. E-mail: gqyu{at}iris.sipp.ac.cn.

Journal of Bacteriology, July 2000, p. 3920-3923, Vol. 182, No. 14
0021-9193/00/$04.00+0
Copyright © 2000, American Society for Microbiology. All rights reserved.


This article has been cited by other articles:

  • Yee, N., Ma, J., Dalia, A., Boonfueng, T., Kobayashi, D. Y. (2007). Se(VI) Reduction and the Precipitation of Se(0) by the Facultative Bacterium Enterobacter cloacae SLD1a-1 Are Regulated by FNR. Appl. Environ. Microbiol. 73: 1914-1920 [Abstract] [Full Text]  
  • Egener, T., Sarkar, A., Martin, D. E., Reinhold-Hurek, B. (2002). Identification of a NifL-like protein in a diazotroph of the {beta}-subgroup of the Proteobacteria, Azoarcus sp. strain BH72. Microbiology 148: 3203-3212 [Abstract] [Full Text]  


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