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Journal of Bacteriology, July 2000, p. 3929-3933, Vol. 182, No. 14
0021-9193/00/$04.00+0
Copyright © 2000, American Society for Microbiology. All rights reserved.

An Abundant DNA Binding Protein from the Hyperthermophilic Archaeon Sulfolobus shibatae Affects DNA Supercoiling in a Temperature-Dependent Fashion

Hong Xue, Rong Guo, Yunfei Wen, Danxu Liu, and Li Huang*

State Key Laboratory of Microbial Resources, Institute of Microbiology, Chinese Academy of Sciences, Beijing 100080, People's Republic of China

Received 20 December 1999/Accepted 4 May 2000

The DNA binding protein Ssh10b, a member of the Sac10b family, has been purified from the hyperthermophilic archaeon Sulfolobus shibatae. Ssh10b constitutes about 4% of the cellular protein. Electrophoretic mobility shift assays showed that Ssh10b first bound a double-stranded DNA fragment with an estimated binding size of ~~12 bp, forming distinct shifts, until the DNA was coated with the protein. Binding of more Ssh10b resulted in the formation of smears of lower mobilities. The migration pattern of the smearing Ssh10b-DNA complexes was affected by temperature, whereas that of complexes associated with the distinct shifts was not. Interestingly, Ssh10b was capable of constraining negative DNA supercoils in a temperature-dependent fashion. While the ability of the protein to constrain supercoils was weak at 25°C, it was enhanced substantially at 45°C or higher temperatures (up to 80°C). Taken together, our data suggest that archaeal proteins of the Sac10b family may affect the topology of chromosomal DNA in thermophilic archaea at their growth temperatures.

* Corresponding author. Mailing address: State Key Laboratory of Microbial Resources, Institute of Microbiology, Chinese Academy of Sciences, Beijing 100080, People's Republic of China. Phone: 86-10-62624971. Fax: 86-10-62560912. E-mail: huangl{at}sun.im.ac.cn.

Journal of Bacteriology, July 2000, p. 3929-3933, Vol. 182, No. 14
0021-9193/00/$04.00+0
Copyright © 2000, American Society for Microbiology. All rights reserved.


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