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Journal of Bacteriology, July 2000, p. 3998-4004, Vol. 182, No. 14
0021-9193/00/$04.00+0
Copyright © 2000, American Society for Microbiology. All rights reserved.

A Novel Bacterial ATP-Binding Cassette Transporter System That Allows Uptake of Macromolecules

Keiko Momma,* Masako Okamoto, Yumiko Mishima, Shigetarou Mori, Wataru Hashimoto, and Kousaku Murata

Research Institute for Food Science, Kyoto University, Uji, Kyoto 611-0011, Japan

Received 1 March 2000/Accepted 2 May 2000

A gram-negative bacterium, Sphingomonas sp. strain A1, isolated as a producer of alginate lyase, has a characteristic cell envelope structure and forms a mouth-like pit on its surface. The pit is produced only when the cells have to incorporate and assimilate alginate. An alginate uptake-deficient mutant was derived from cells of strain A1. One open reading frame, algS (1,089 bp), exhibiting homology to the bacterial ATP-binding domain of an ABC transporter, was cloned as a fragment complementing the mutation. algS was followed by two open reading frames, algM1 (972 bp) and algM2 (879 bp), which exhibit homology with the transmembrane permeases of ABC transporters. Disruption of algS of strain A1 resulted in the failure to incorporate alginate and to form a pit. Hexahistidine-tagged AlgS protein (AlgSHis6) overexpressed in Escherichia coli and purified by Ni2+ affinity column chromatography showed ATPase activity. Based on these results, we propose the occurrence of a novel pit-dependent ABC transporter system that allows the uptake of macromolecules.

* Corresponding author. Mailing address: Research Institute for Food Science, Kyoto University, Uji, Kyoto 611-0011, Japan. Phone: 81-774-38-3768. Fax: 81-774-38-3767. E-mail: momma{at}food2.food.kyoto-u.ac.jp.

Journal of Bacteriology, July 2000, p. 3998-4004, Vol. 182, No. 14
0021-9193/00/$04.00+0
Copyright © 2000, American Society for Microbiology. All rights reserved.


This article has been cited by other articles:

  • Miyake, O., Ochiai, A., Hashimoto, W., Murata, K. (2004). Origin and Diversity of Alginate Lyases of Families PL-5 and -7 in Sphingomonas sp. Strain A1. J. Bacteriol. 186: 2891-2896 [Abstract] [Full Text]  
  • Nikaido, H. (2003). Molecular Basis of Bacterial Outer Membrane Permeability Revisited. Microbiol. Mol. Biol. Rev. 67: 593-656 [Abstract] [Full Text]  
  • Mishima, Y., Momma, K., Hashimoto, W., Mikami, B., Murata, K. (2003). Crystal Structure of AlgQ2, a Macromolecule (Alginate)-binding Protein of Sphingomonas sp. A1, Complexed with an Alginate Tetrasaccharide at 1.6-A Resolution. J. Biol. Chem. 278: 6552-6559 [Abstract] [Full Text]  
  • Hashimoto, W., Miyake, O., Momma, K., Kawai, S., Murata, K. (2000). Molecular Identification of Oligoalginate Lyase of Sphingomonas sp. Strain A1 as One of the Enzymes Required for Complete Depolymerization of Alginate. J. Bacteriol. 182: 4572-4577 [Abstract] [Full Text]  


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